Periplakin Antibody

Western Blot: Periplakin Antibody [NBP2-19775] - Sample (30 ug of whole cell lysate) A: U87-MG 5% SDS PAGE gel, diluted at 1:5000.

Immunohistochemistry-Paraffin: Periplakin Antibody [NBP2-19775] - Paraffin-embedded mouse prostate. Periplakin antibody [N1N2], N-term dilution: 1:500.

Immunohistochemistry-Paraffin: Periplakin Antibody [NBP2-19775] - Paraffin-embedded U87 xenograft. Periplakin antibody [N1N2], N-term dilution: 1:500.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Periplakin Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Periplakin. The exact sequence is proprietary.
• Localization: Cell junction; desmosome; Cytoplasm; cytoskeleton; Cell membrane (By similarity); Nucleus (By similarity); Mitochondrion (By similarity)
• Predicted Species: Rat (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Theoretical MW: 205 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Periplakin Antibody

• 190 kDa paraneoplastic pemphigus antigen
• 195 kDa cornified envelope precursor protein
• KIAA0568
• MGC134872
• periplakin

Background

The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PPL
• Uniprot:
  • O60437(Human)