Periplakin Antibody

Images

 
Western Blot: Periplakin Antibody [NBP2-19775] - Sample (30 ug of whole cell lysate) A: U87-MG 5% SDS PAGE gel, diluted at 1:5000.
Immunohistochemistry-Paraffin: Periplakin Antibody [NBP2-19775] - Paraffin-embedded mouse prostate. Periplakin antibody [N1N2], N-term dilution: 1:500.
Immunohistochemistry-Paraffin: Periplakin Antibody [NBP2-19775] - Paraffin-embedded U87 xenograft. Periplakin antibody [N1N2], N-term dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Periplakin Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Periplakin. The exact sequence is proprietary.
Localization
Cell junction; desmosome; Cytoplasm; cytoskeleton; Cell membrane (By similarity); Nucleus (By similarity); Mitochondrion (By similarity)
Predicted Species
Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
205 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Periplakin Antibody

  • 190 kDa paraneoplastic pemphigus antigen
  • 195 kDa cornified envelope precursor protein
  • KIAA0568
  • MGC134872
  • periplakin

Background

The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol PPL
Uniprot