Periplakin Antibody

Western Blot: Periplakin Antibody [NB100-55264] - Detection of Mouse and Rat Periplakin/PPL by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-Periplakin/PPL antibody NB100-55264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: Periplakin Antibody [NB100-55264] - Detection of Human Periplakin/PPL on HeLa whole cell lysate using NB100-55264. Periplakin/PPL was also immunoprecipitated by another rabbit anti-Periplakin/PPL antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Periplakin Antibody Summary

• Description: Novus Biologicals Rabbit Periplakin Antibody (NB100-55264) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1150 and 1200 of human periplakin using the numbering given in entry NP_002696.3 (GeneID 5493).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 mcg/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Periplakin Antibody

• 190 kDa paraneoplastic pemphigus antigen
• 195 kDa cornified envelope precursor protein
• KIAA0568
• MGC134872
• periplakin

Background

Peroplakin is a component of the cornified envelope of keratinocytes. It may link the cornified envelope to desmosomes and intermediate filaments and may act as a localization signal in PKB/AKT-mediated signaling. Endogenous periplakin localizes to different cellular compartments, including plasma membrane, intermediate filament structures, the nucleus and mitochondria. Overexpression of the C-terminal part of periplakin, encompassing the PKB binding region, results in predominant intermediate filament localization and little nuclear staining. Periplakin is expressed in epithelial cells, together with envoplakin, the precursor of the epidermal cornified envelope. Furthermore, periplakin has been shown to be a target antigen in paraneoplastic pemphigus, and in keratinocytes it localizes at desmosomes and the interdesmosomal plasma membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPL
• Entrez:
  • 5493(Human)
• Uniprot:
  • O60437(Human)