Perilipin-3/TIP47 Antibody [DyLight 488]

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 488

Perilipin-3/TIP47 Antibody [DyLight 488] Summary

• Immunogen: A synthetic peptide made to a region within the N-terminus (within residues 1-100) of the mouse TIP47 protein. [Swiss-Prot# Q9DBG5]
• Localization: Cytoplasm. Endosome. Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLIN3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Western Blot
• Application Notes: This TIP47 antibody is useful for Western Blot and Immunocytochemistry/Immunofluorescence. In Western Blot analysis, a band is seen ~47 kDa (isoform B, containing the first 184 residues that isoform A is lacking). In ICC/IF, endosomal staining was observed in U2OS cells.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Perilipin-3/TIP47 Antibody [DyLight 488]

• Cargo selection protein TIP47
• M6PRBP1
• M6PRBP1MGC2012
• Mannose-6-phosphate receptor-binding protein 1
• perilipin 3,47 kDa MPR-binding protein
• Perilipin3
• Perilipin-3
• Placental protein 17,47 kDa mannose 6-phosphate receptor-binding protein
• PLIN3
• PP17
• PP17MGC11117
• tail-interacting protein, 47 kD
• TIP47
• TIP47mannose-6-phosphate receptor binding protein 1

Background

TIP47 has been described as cargo protein involved in the trafficking of the mannose-6-phosphate receptor between endosomes and the Golgi complex. Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. TIP47 has been localized in milk fat globule membranes of human and bovine origin. It has been described as a placental protein, as well. Increased amounts of TIP47 are secreted into circulation of cervix carcinoma patients. Therefore, this protein is probably significant as an oncodevelopmental marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLIN3