Peptidase Inhibitor 16/PI16 Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Peptidase Inhibitor 16/PI16 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Peptidase Inhibitor 16/PI16
  Leu28-Leu437
  Accession # AAH35634
• Specificity: Detects human Peptidase Inhibitor 16/PI16 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PI16
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Peptidase Inhibitor 16/PI16 Antibody [Unconjugated]

• CRISP9
• CRISP-9
• Cysteine-rich secretory protein 9
• dJ90K10.5
• DKFZp586B1817
• MGC45378
• microseminoprotein, beta-binding protein
• MSMBBP
• Peptidase Inhibitor 16
• PI16
• PI-16
• protease inhibitor 16
• PSP94-binding protein
• PSPBP

Background

PI16 (Peptidase Inhibitor 16; also PSP94-binding protein) is a 70‑75 kDa member of the CRISP family of proteins. It is expressed by prostatic fibroblasts and cardiomyocytes and is known to bind PSP94. The human PI16 precursor is 463 amino acids (aa) in length. It contains a 27 aa signal peptide plus a 436 aa mature region. The mature region has been described as being either a type I transmembrane (TM) glycoprotein with a TM segment between aa 443‑462, or a GPI‑linked glycoprotein with C‑terminal cleavage between Lys437‑Asn438. Three potential variants exist. One shows a deletion of aa 231‑423, a second shows a 9 aa substitution for the C‑terminal 64 aa and a third shows a 43 aa insertion after Cys197. Over aa 28‑437, human PI16 is 67% aa identical to mouse PI16.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Peptidase Inhibitor 16/PI16 Antibody (AF4980)(2)

Publications using AF4980

• Chen, Z;Huang, Z;Kim, WS;Debnath, R;Prasongyuenyong, K;DerGarabedian, BP;Do, T;Moore-Kosslow, MA;Korostoff, JM;Chen, C;Choi, Y;Ko, KI; Fibroblast-derived alarmin promotes oral wound healing by activating regulatory T cells that relay pro-angiogenic and anti-inflammatory responses Cell reports 2026-01-13 [PMID: 41533514] (Immunohistochemistry, Human)
• Calyeca, J;Hussein, Z;Tan, ZH;Liu, L;Dharmadhikari, S;Shontz, KM;Vetter, TA;Breuer, CK;Reynolds, SD;Chiang, T; Orchestrated response from heterogenous fibroblast subsets contributes to repair from surgery-induced stress after airway reconstruction JCI insight 2025-01-21 [PMID: 39836476] (Immunohistochemistry, Mouse)

Bioinformatics

• Gene Symbol: PI16
• Uniprot:
  • AAH35634()