CRISPLD2 Antibody - BSA Free

Western Blot: CRISPLD2 Antibody [NBP1-85143] - Analysis in human cell line BEWO.

Immunohistochemistry-Paraffin: CRISPLD2 Antibody [NBP1-85143] - Staining of human duodenum shows strong granular positivity in glandular cells.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CRISPLD2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKAKIFGSLFYESSS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRISPLD2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  CRISPLD2 Protein (NBP1-85143PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31746554). Rat (83%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CRISPLD2 Antibody - BSA Free

• CRISP11
• CRISP-11
• Cysteine-rich secretory protein 11
• cysteine-rich secretory protein LCCL domain containing 2
• cysteine-rich secretory protein LCCL domain-containing 2
• DKFZP434B044
• LCCL domain containing cysteine-rich secretory protein 2
• LCCL domain-containing cysteine-rich secretory protein 2
• LCRISP2
• MGC74865

Background

Promotes matrix assembly

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRISPLD2 Antibody (NBP1-85143)(3)

Publications using NBP1-85143

• Jackson RM, Griesel BA, Short KR et al. Weight Loss Results in Increased Expression of Anti-Inflammatory Protein CRISPLD2 in Mouse Adipose Tissue Obesity (Silver Spring) 2019-12-01 [PMID: 31746554] (WB, Mouse)
• Yoo JY, Shin H, Kim TH et al. CRISPLD2 Is a Target of Progesterone Receptor and Its Expression Is Decreased in Women with Endometriosis. PLoS One 2014-01-01 [PMID: 24955763] (WB, IF/IHC, Human)
• Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261]

Review for CRISPLD2 Antibody (NBP1-85143) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human and Mouse.

reviewed by: Mei Deng IHC-P Human and Mouse 02/24/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Adult lung,Embryonic lung
• Species: Human and Mouse

Comments

• Comments: Nice staining of IHC-P on human and mouse tissue with this antibody at a dilution 1:500. The section was pre-treated by heat mediated antigen retrieval with sodium citrate buffer (pH6).

Bioinformatics

• Gene Symbol: CRISPLD2