RTVP-1/GLIPR1 Antibody

Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Mouse RTVP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4468) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RTVP-1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

RTVP-1/GLIPR1 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse RTVP-1
  Ser16-Thr223
  Accession # Q9CWG1
• Specificity: Detects mouse RTVP-1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GLIPR1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RTVP-1/GLIPR1 Antibody

• GLI pathogenesis-related 1 (glioma)
• GLI pathogenesis-related 1
• GliPR 1
• GliPR
• GLIPR1
• GLIPRglioma pathogenesis-related protein 1
• Protein RTVP-1
• related to testis-specific, vespid, and pathogenesis proteins 1
• RTVP1
• RTVP-1
• RTVP1CRISP7
• testes-specific vespid and pathogenesis protein 1

Background

RTVP-1 (related to testes-specific, vespid, and pathogenesis protein 1) can act as both a tumor suppressor and tumor promoter gene. RTVP-1 is a tumor suppressor gene based on its regulation by p53, induction of apoptosis, growth inhibition, and decreased expression in prostate tumors when compared to normal prostate. RTVP-1 is a tumor promoter is based on evidence that overexpression of RTVP-1 decreased the phosphorylation of c-Jun, increased Bcl-2 expression, and increased MMP2 activity. In addition, RTVP-1 is expressed at high levels in glioblastomas while barely detectable in normal brain/astrocytes. Differences in the levels of cellular and extracellular forms as well as different cellular processing of RTVP-1 may account for these differing roles. Additionally, increased RTVP-1 expression is associated with myelomocytic differentiation in macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RTVP-1/GLIPR1 Antibody (AF4468)(1)

Publication using AF4468

• N Friend, JE Noll, KS Opperman, KC Clark, KM Mrozik, K Vandyke, DR Hewett, ACW Zannettino GLIPR1 expression is reduced in multiple myeloma but is not a tumour suppressor in mice PLoS ONE, 2020-01-29;15(1):e0228408. 2020-01-29 [PMID: 31995627] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: GLIPR1
• Uniprot:
  • Q9CWG1()