GLIPR2 Antibody - BSA Free

Western Blot: GLIPR2 Antibody [NBP2-47126] - Total protein from human liver (lane 1) and human spleen (lane 2) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-GAPR1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry-Paraffin: GLIPR2 Antibody [NBP2-47126] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using GLIPR2 antibody at 1:200 dilution with HRP-DAB detection and hematoxylin counterstaining. GLIPR2 is a protein which localizes to Golgi membrane as well as extracellular exosomes, and this GLIPR2 antibody generated a very specific cytoplasmic staining with strong signal in extracellular spaces/around cellular membranes.

Immunohistochemistry-Paraffin: GLIPR2 Antibody [NBP2-47126] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using GLIPR2 antibody at 1:200 dilution with HRP-DAB detection and hematoxylin counterstaining. GLIPR2 is a protein which localizes to Golgi membrane as well as extracellular exosomes, and this GLIPR2 antibody generated a very specific cytoplasmic staining with strong signal in extracellular spaces/around cellular membranes.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GLIPR2 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to a C-terminal portion of the human GAPR1 protein (between amino acids 90-154) [UniProt Q9H4G4]
• Localization: Golgi membrane and extracellular exosomes
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GLIPR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1: 200 - 1:500
  • Immunohistochemistry-Paraffin 1: 200 - 1:500
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GLIPR2 Antibody - BSA Free

• C9orf19Glioma pathogenesis-related protein 2
• chromosome 9 open reading frame 19
• GAPR1
• GAPR-117kD fetal brain protein
• GLI pathogenesis-related 2
• GliPR 2
• Golgi-associated plant pathogenesis-related protein 1
• Golgi-associated PR-1 protein

Background

GLIPR2 is closely related to plant pathogenesis-related (PR-1) proteins, which are upregulated in response to pathogen attack. This protein is found within lipid-enriched microdomains on the cytosolic side of the endomembrane system. GLIPR2 is tightly anchored to membranes and absent from the cytosol, although it does not possess a membrane-spanning domain. Recombinant human GLIPR2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLIPR2
• Uniprot:
  • Q9H4G4(Human)