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GLIPR2 Antibody

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Western Blot: GLIPR2 Antibody [NBP2-47126] - Total protein from human liver (lane 1) and human spleen (lane 2) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-GAPR1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Western Blot: GLIPR2 Antibody [NBP2-47126] - Total protein from human liver (lane 1) and human spleen (lane 2) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat ...read more
Immunohistochemistry-Paraffin: GLIPR2 Antibody [NBP2-47126] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using GLIPR2 antibody at 1:200 dilution with HRP-DAB detection and ...read more
Immunohistochemistry-Paraffin: GLIPR2 Antibody [NBP2-47126] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using GLIPR2 antibody at 1:200 dilution with HRP-DAB detection and ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47126AF350
Alexa Fluor 405
NBP2-47126AF405
Alexa Fluor 488
NBP2-47126AF488
Alexa Fluor 532
NBP2-47126AF532
Alexa Fluor 594
NBP2-47126AF594
Alexa Fluor 647
NBP2-47126AF647
Alexa Fluor 700
NBP2-47126AF700
Alexa Fluor 750
NBP2-47126AF750
Biotin
NBP2-47126B
DyLight 350
NBP2-47126UV
DyLight 405
NBP2-47126V
DyLight 488
NBP2-47126G
DyLight 550
NBP2-47126R
DyLight 594
NBP2-47126DL594
DyLight 650
NBP2-47126C
DyLight 680
NBP2-47126FR
DyLight 755
NBP2-47126IR
HRP
NBP2-47126H
Janelia Fluor 549
NBP2-47126JF549
Janelia Fluor 646
NBP2-47126JF646
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GLIPR2 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human GAPR1 protein (between amino acids 90-154) [UniProt Q9H4G4]
Localization
Golgi membrane and extracellular exosomes
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLIPR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:1000
  • Immunohistochemistry 1: 200 - 1:500
  • Immunohistochemistry-Paraffin 1: 200 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GLIPR2 Antibody

  • C9orf19Glioma pathogenesis-related protein 2
  • chromosome 9 open reading frame 19
  • GAPR1
  • GAPR-117kD fetal brain protein
  • GLI pathogenesis-related 2
  • GliPR 2
  • Golgi-associated plant pathogenesis-related protein 1
  • Golgi-associated PR-1 protein

Background

GLIPR2 is closely related to plant pathogenesis-related (PR-1) proteins, which are upregulated in response to pathogen attack. This protein is found within lipid-enriched microdomains on the cytosolic side of the endomembrane system. GLIPR2 is tightly anchored to membranes and absent from the cytosol, although it does not possess a membrane-spanning domain. Recombinant human GLIPR2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Caveolin-1 Antibody (7C8) [NB100-615] - Detection of caveolin in 3T3 cell lysates (50 ug). Lanes 1 and 2: 1:4000. Lanes 3 and 4: 1:1000. Detection by ECL: 5 minute exposure.
Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Caveolin-1 [7C8] conjugated to Alexa Fluor 488 (NB100-615AF488) at 20 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.
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Western Blot: ITK Antibody (5G6) [NBP2-37578] - Analysis using ITK mouse mAb against Jurkat cell lysate.
Immunocytochemistry/Immunofluorescence: ITK Antibody (5G6) [NBP2-37578] - Immunofluorescence analysis of Jurkat cells using ITK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry: ITK Antibody (5G6) [NBP2-37578] - Analysis of Jurkat cells using ITK mouse mAb (blue) and negative control (red).
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Carbonic Anhydrase VA/CA5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3049) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Carbonic Anhydrase VA/CA5A at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Tumstatin Monoclonal Antibody (Catalog # MAB7546) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tumstatin at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Western Blot: Caveolin-2 Antibody [NBP1-31116] - Rat tissue extracts (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: Caveolin-2 Antibody [NBP1-31116] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Caveolin 2 protein stained by Caveolin 2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
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Western Blot: Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates. Antibody at 1:5000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5000 in green, revealing multiple bands around 280 kDa that correspond to full length MAP2A/2B isotypes, and ~70 kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.
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Immunohistochemistry-Frozen: Vimentin Antibody [NB300-223] - Infarcted mouse heart frozen sections stained with vimentin antibody at 1:200. Secondary antibody at 1:500. Fixation with 4% PFA. IHC-Fr image submitted by a verified customer review.
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Immunohistochemistry-Paraffin: PTHLH/PTHrP Antibody [NBP1-59322] - Human Lung Alveolar cells (indicated with arrows), 4-8ug/ml.
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GLIPR2 Antibody
NBP2-47126
GLIPR2 Antibody
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Applications: WB, IHC, IHC-P

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47126AF350
Alexa Fluor 405 NBP2-47126AF405
Alexa Fluor 488 NBP2-47126AF488
Alexa Fluor 532 NBP2-47126AF532
Alexa Fluor 594 NBP2-47126AF594
Alexa Fluor 647 NBP2-47126AF647
Alexa Fluor 700 NBP2-47126AF700
Alexa Fluor 750 NBP2-47126AF750
Biotin NBP2-47126B
DyLight 350 NBP2-47126UV
DyLight 405 NBP2-47126V
DyLight 488 NBP2-47126G
DyLight 550 NBP2-47126R
DyLight 594 NBP2-47126DL594
DyLight 650 NBP2-47126C
DyLight 680 NBP2-47126FR
DyLight 755 NBP2-47126IR
HRP NBP2-47126H
Janelia Fluor 549 NBP2-47126JF549
Janelia Fluor 646 NBP2-47126JF646
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Bioinformatics

Gene Symbol GLIPR2
Uniprot
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