Peptidase Inhibitor 16/PI16 Antibody [Unconjugated]

Mouse pancreas cryosections stained with CD324 (green) and PI16 (white). Image from a verified customer review.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Peptidase Inhibitor 16/PI16 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Peptidase Inhibitor 16/PI16
  Leu21-Glu464
  Accession # Q9ET66
• Specificity: Detects mouse Peptidase Inhibitor 16/PI16 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PI16
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Peptidase Inhibitor 16/PI16 Antibody [Unconjugated]

• CRISP9
• CRISP-9
• Cysteine-rich secretory protein 9
• dJ90K10.5
• DKFZp586B1817
• MGC45378
• microseminoprotein, beta-binding protein
• MSMBBP
• Peptidase Inhibitor 16
• PI16
• PI-16
• protease inhibitor 16
• PSP94-binding protein
• PSPBP

Background

PI16 (Peptidase Inhibitor 16) is a variable molecular weight (MW) member of the CRISP family of proteins. It is expressed by cardiomyocytes and serves as an autocrine negative growth regulator. Mature mouse PI16 is a type I transmembrane protein 469 amino acids (aa) in length. The extracellular region includes all but the C‑terminal 21 amino acids. A soluble form of PI16 is known. Although its predicted MW is 50 kDa, it runs anomalously at 70 kDa (unmodified) in SDS-PAGE. With glycosylation, it appears to be 100‑110 kDa in size. Three potential variants exist. One shows a deletion of aa 51‑124, a second shows a 9 aa extension at the N‑terminus, and a third shows a deletion of aa 191‑452. Over aa 1‑464, mouse PI16 shares 86% and 61% aa identity with rat and human PI16, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Peptidase Inhibitor 16/PI16 Antibody (AF4929)(6)

Publications using AF4929

• Fang, Y;Chung, SSW;Xu, L;Xue, C;Liu, X;Jiang, D;Li, R;Korogi, Y;Yuan, K;Saqi, A;Hibshoosh, H;Huang, Y;Lin, CS;Tsukui, T;Sheppard, D;Sun, X;Que, J; RUNX2 promotes fibrosis via an alveolar-to-pathological fibroblast transition Nature 2025-02-05 [PMID: 39910313] (Immunohistochemistry, Mouse)
• Lee, JY;Reyes, N;Woo, SH;Goel, S;Stratton, F;Kuang, C;Mansfield, AS;LaFave, LM;Peng, T; Senescent fibroblasts in the tumor stroma rewire lung cancer metabolism and plasticity bioRxiv : the preprint server for biology 2024-07-30 [PMID: 39131266] (Immunohistochemistry, Transgenic Mouse)
• Garrity, R;Arora, N;Areeful Haque, M;Weis, D;Trinh, RT;Neerukonda, SV;Kumari, S;Cortez, I;Ubogu, EE;Mahalingam, R;Tavares-Ferreira, D;Price, TJ;Kavelaars, A;Heijnen, CJ;Shepherd, AJ; Fibroblast-derived PI16 sustains inflammatory pain via regulation of CD206+ myeloid cells Brain, behavior, and immunity 2023-06-12 [PMID: 37315702] (IHC, Western Blot, Mouse)
• Pooja Singhmar, Ronnie The Phong Trinh, Jiacheng Ma, XiaoJiao Huo, Bo Peng, Cobi J. Heijnen et al. The fibroblast-derived protein PI16 controls neuropathic pain Proceedings of the National Academy of Sciences 3/10/2020 [PMID: 32079726]
• Chen J, Walton K, Winbanks C, Murphy K, Thomson R, Makanji Y, Qian H, Lynch G, Harrison C, Gregorevic P Elevated expression of activins promotes muscle wasting and cachexia. FASEB J, 2013-12-30;28(4):1711-23. 2013-12-30 [PMID: 24378873] (Western Blot, Mouse)
• Ghosh , Asish K, Murphy , Sheila B, Kishore , Raj, Vaughan , Douglas Global gene expression profiling in PAI-1 knockout murine heart and kidney: molecular basis of cardiac-selective fibrosis. PLoS ONE, 2013-05-28;8(5):e63825. 2013-05-28 [PMID: 23724005] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: PI16
• Uniprot:
  • Q9ET66()