PELP1 Antibody

Immunohistochemistry-Paraffin: PELP1 Antibody [NB110-40622] - Section of human testicular seminoma. Antibody: Affinity purified rabbit antiPELP1/MNAR used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.

Immunoprecipitation: PELP1 Antibody [NB110-40622] - Detection of Human PELP1/MNAR by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PELP1/MNAR antibody NB110-40622 used for IP at 3 mcg/mg lysate. PELP1/MNAR was also immunoprecipitated by other rabbit anti-PELP1/MNAR antibodies, which recognize downstream epitopes. Detection: Chemiluminescence with an exposure time of 1 second.

• Reactivity: Hu, Pm
• Applications: IHC, IHC-P, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

PELP1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human Proline-, Glutamic Acid-, Leucine-Rich Protein 1 (Modulator of Nongenomic Activity of Estrogen Receptor) using the numbering given in entry AAN41255.1 (GeneID 27
• Predicted Species: Monkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PELP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500- 1:2000
  • Immunohistochemistry-Paraffin 1:500- 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PELP1 Antibody

• HMX3
• MNARmodulator of nongenomic activity of estrogen receptor
• Modulator of non-genomic activity of estrogen receptor
• P160
• proline and glutamic acid rich nuclear protein
• proline, glutamate and leucine rich protein 1
• proline, glutamic acid and leucine rich protein 1
• proline-, glutamic acid- and leucine-rich protein 1
• proline-, glutamic acid-, leucine-rich protein 1
• Transcription factor HMX3

Background

PELP1/MNAR was identified as an SH2 domain interacting protein that interacts with Lck, c-Src, and other members of the Src kinase family. PELP1/MNAR appears to function as a general coregulator and interacts with multiple nuclear receptors and transcription factors. PELP1/MNAR has also been shown to participate in nongenomic signaling via an association with a multiprotein complex, the

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PELP1 Antibody (NB110-40622)(2)

Publications using NB110-40622

• Fanis P, Gillemans N, Aghajanirefah A et al. Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation. Mol Cell Proteomics 2012-11-01 [PMID: 22872859]
• Habashy HO, Rakha EA, Aleskandarany M et al. FOXO3a nuclear localisation is associated with good prognosis in luminal-like breast cancer. Breast Cancer Res Treat 129(1):11-21. 2011-08-01 [PMID: 21336599]

Bioinformatics

• Gene Symbol: PELP1
• Entrez:
  • 27043(Human)
• Uniprot:
  • AAN41255.1(Human)