PDI Antibody

Images

 
Western Blot: P4HB Antibody [NBP1-97470] - Analysis of PDI: Lane 1: MWM, Lane 2: P4HB (human), (recombinant) (His-tag) (Prod. No.ADI-SPP-891), Lane 3: HeLa, (cell lysate), Lane 4: Rat Testis Tissue Lysate, Lane 5: Mouse ...read more
Immunohistochemistry-Paraffin: P4HB Antibody [NBP1-97470] - Analysis of human spleen tissue stained with P4HB, pAb at 10ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, GP, Ha, Pm, Sh, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PDI Antibody Summary

Immunogen
Purified bovine liver PDI.
Marker
Endoplasmic Reticulum Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDIA2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
This antibody is useful in Immunocytochemistry, Immunoprecipitation, Immunohistochemistry (paraffin sections) and detects a band of ~58kDa by Western blot.
Publications
Read Publications using
NBP1-97470 in the following applications:

  • 4 publications
  • IP
    2 publications
  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for PDI Antibody

  • Pancreas-specific protein disulfide isomerase
  • pancreatic protein disulfide isomerase
  • PDA2
  • PDI
  • PDIp
  • PDIPEC 5.3.4.1
  • PDIR
  • protein disulfide isomerase A2
  • protein disulfide isomerase family A, member 2
  • protein disulfide isomerase, pancreatic
  • protein disulfide isomerase-associated 2
  • protein disulfide-isomerase A2
  • Rho GDP dissociation inhibitor gamma

Background

The mammalian PDI (Protein disulfide-isomerase) family encompasses several highly divergent proteins which are involved in the processing and maturation of secretory proteins in the endoplasmic reticulum by catalyzing the rearrangement of disulfide bonds.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDI Antibody (NBP1-97470)(10)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Porcine, Primate, Sheep.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(4)
IP
(2)
WB
(4)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
Rat
(3)
Porcine
(1)
Primate
(1)
Sheep
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using NBP1-97470 Applications Species
S Lindquist et al. Impaired ERAD and ER stress are early and specific events in polyglutamine toxicity. Genes Dev. 22, 3308. 2008 [PMID: 19015277] (WB, Human) WB Human
K Brix et al. Trafficking of lysosomal cathepsin B-green fluorescent protein to the surface of thyroid epithelial cells involves the endosomal/lysosomal compartment. J. Cell. Sci. 115, 4877. 2002 [PMID: 12432075] (ICC/IF, Rat) ICC/IF Rat
DE Vance et al. Membrane topography of human phosphatidylethanolamine N-methyltransferase. J. Biol. Chem. 278, 2956. 2003 [PMID: 12431977] (WB, Mouse) WB Mouse
AH Futerman et al. Upstream of growth and differentiation factor 1 (uog1), a mammalian homolog of the yeast longevity assurance gene 1 (LAG1), regulates N-stearoyl-sphinganine (C18-(dihydro)ceramide) synthesis in a fumonisin B1-independent manner in mammalian cells. J. Biol. Chem. 277, 35642. 2002 [PMID: 12105227] (ICC/IF, Primate) ICC/IF Primate
J Dean et al. Conserved furin cleavage site not essential for secretion and integration of ZP3 into the extracellular egg coat of transgenic mice. Mol. Cell. Biol. 22, 3111. 2002 [PMID: 11940668] (ICC/IF, Mouse) ICC/IF Mouse
G Warren et al. Partitioning of the matrix fraction of the Golgi apparatus during mitosis in animal cells. Science 295, 848. 2002 [PMID: 11823640] (WB, Rat) WB Rat
MH Doolittle et al. Maturation of lipoprotein lipase in the endoplasmic reticulum: concurrent formation of functional dimers and inactive aggregates. J. Biol. Chem. 277, 10727. 2002 [PMID: 11796709] (IP, Human) IP Human
I Merida et al. Type I alpha phosphatidylinositol 4-phosphate 5-kinase is a putative target for increased intracellular phosphatidic acid. FEBS Lett. 476, 160. 2000 [PMID: 10913605] (ICC/IF, Porcine) ICC/IF Porcine
P Klappa et al. Specificity in substrate binding by protein folding catalysts: tyrosine and tryptophan residues are the recognition motifs for the binding of peptides to the pancreas-specific protein disulfide isomerase PDIp. Protein Sci. 9, 758. 2000 [PMID: 10794419] (WB, Sheep) WB Sheep
R Miquelis et al. Protein-disulfide isomerase (PDI) in FRTL5 cells - pH-dependent thyroglobulin/PDI interactions determine a novel PDI function in the post-endoplasmic reticulum of thyrocytes. J. Biol. Chem. 275, 1920. 2000 [PMID: 10636893] (IP, Rat) IP Rat

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDI Antibody (NBP1-97470) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for PDI Antibody (NBP1-97470)

Discover related pathways, diseases and genes to PDI Antibody (NBP1-97470). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on PDI.

ERO1 Activity: A Potential Source of ER-Derived Oxidative Stress.
Disulfide bond formation is a pivotal step in the maturation and release of secretory proteins that are controlled by specific endoplasmic reticulum (ER) resident enzymes. An important element in this process is ERO (ER oxidoreduction), a glycosylated...  Read full blog post.

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Calculators

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Bioinformatics

Gene Symbol PDIA2