PDHX Antibody

Western Blot: PDHX Antibody [NB100-1496] - Western blot analysis of Human Pancreas lysate (35ug protein in RIPA buffer) using NB100-1496 (1ug/ml).

Immunohistochemistry: PDHX Antibody [NB100-1496] - staining of paraffin embedded Human Pancreas. A) Microwaved antigen retrieval with citrate buffer pH 6 (3ug/ml), HRP-staining. B) Microwaved antigen retrieval with Tris/EDTA buffer pH9 (1ug/ml) , HRP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PDHX Antibody Summary

• Immunogen: Peptide with sequence C-KSFKANLENPIRLA corresponding to C-Terminus according to NP_003468.2, NP_001128496.1, NP_001159630.1.
• Specificity: This antibody is expected to recognise all reported isoforms.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDHX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 1 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in human pancreas lysate (calculated MW of 54.2 kDa band according to NP_003468.1). In transfected HEK293 transiently expressing PDHX a band of approx. 50 kDa is observed. This band is not observed in the non-transfected HEK293.
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  PDHX Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDHX Antibody

• Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenasecomplex
• DLDBP
• E3-binding protein
• E3BP
• E3BP1
• E3BPpyruvate dehydrogenase complex, E3-binding protein subunit
• OPDX
• PDHX
• PDX1
• PDX1Lipoyl-containing pyruvate dehydrogenase complex component X
• proX
• proXpyruvate dehydrogenase complex, lipoyl-containing component X
• pyruvate dehydrogenase complex, component X
• pyruvate dehydrogenase protein X component, mitochondrial

Background

The PDHX gene encodes component X of the pyruvate dehydrogenase (PDH) complex. For a detailed description of the pyruvate dehydrogenase complex, see MIM 300502. The mammalian PDH complex differs from that in E. coli and from the other mammalian alpha-keto acid dehydrogenases by the presence of a 53-kD protein called protein X. Component X binds to the E3 (MIM 238331) component of the PDH complex (Robinson et al., 1990; Aral et al., 1997).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDHX Antibody (NB100-1496)(1)

Publication using NB100-1496

• Schild M, Ruhs A, Beiter T et al. Basal and exercise induced label-free quantitative protein profiling of m. vastus lateralis in trained and untrained individuals J Proteomics 2015-04-06 [PMID: 25857276] (WB, Human)

Bioinformatics

• Gene Symbol: PDHX
• Entrez:
  • 8050(Human)
  • 27402(Mouse)
• Uniprot:
  • O00330(Human)