Western Blot: PDHX Antibody [NB100-1496] - Western blot analysis of Human Pancreas lysate (35ug protein in RIPA buffer) using NB100-1496 (1ug/ml).
Immunohistochemistry: PDHX Antibody [NB100-1496] - staining of paraffin embedded Human Pancreas. A) Microwaved antigen retrieval with citrate buffer pH 6 (3ug/ml), HRP-staining. B) Microwaved antigen retrieval with ...read more
WB: Approx. 50 kDa band observed in human pancreas lysate (calculated MW of 54.2 kDa band according to NP_003468.1). In transfected HEK293 transiently expressing PDHX a band of approx. 50 kDa is observed. This band is not observed in the non-transfected HEK293.
54 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Tris (pH 7.3), 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for PDHX Antibody
Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenasecomplex
E3BPpyruvate dehydrogenase complex, E3-binding protein subunit
PDX1Lipoyl-containing pyruvate dehydrogenase complex component X
proXpyruvate dehydrogenase complex, lipoyl-containing component X
pyruvate dehydrogenase complex, component X
pyruvate dehydrogenase protein X component, mitochondrial
The PDHX gene encodes component X of the pyruvate dehydrogenase (PDH) complex. For a detailed description of the pyruvate dehydrogenase complex, see MIM 300502. The mammalian PDH complex differs from that in E. coli and from the other mammalian alpha-keto acid dehydrogenases by the presence of a 53-kD protein called protein X. Component X binds to the E3 (MIM 238331) component of the PDH complex (Robinson et al., 1990; Aral et al., 1997).[supplied by OMIM]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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