PDHX Antibody (NB100-1496)

Images

 
Western Blot: PDHX Antibody [NB100-1496] - Western blot analysis of Human Pancreas lysate (35ug protein in RIPA buffer) using NB100-1496 (1ug/ml).
Immunohistochemistry: PDHX Antibody [NB100-1496] - staining of paraffin embedded Human Pancreas. A) Microwaved antigen retrieval with citrate buffer pH 6 (3ug/ml), HRP-staining. B) Microwaved antigen retrieval with ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PDHX Antibody Summary

Immunogen
Peptide with sequence C-KSFKANLENPIRLA corresponding to C-Terminus according to NP_003468.2, NP_001128496.1, NP_001159630.1.
Specificity
This antibody is expected to recognise all reported isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDHX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 1 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 50 kDa band observed in human pancreas lysate (calculated MW of 54.2 kDa band according to NP_003468.1). In transfected HEK293 transiently expressing PDHX a band of approx. 50 kDa is observed. This band is not observed in the non-transfected HEK293.
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
PDHX Lysate (NBL1-14243)
Publications
Read Publication using
NB100-1496 in the following applications:

  • WB
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PDHX Antibody

  • Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenasecomplex
  • DLDBP
  • E3-binding protein
  • E3BP
  • E3BP1
  • E3BPpyruvate dehydrogenase complex, E3-binding protein subunit
  • OPDX
  • PDHX
  • PDX1
  • PDX1Lipoyl-containing pyruvate dehydrogenase complex component X
  • proX
  • proXpyruvate dehydrogenase complex, lipoyl-containing component X
  • pyruvate dehydrogenase complex, component X
  • pyruvate dehydrogenase protein X component, mitochondrial

Background

The PDHX gene encodes component X of the pyruvate dehydrogenase (PDH) complex. For a detailed description of the pyruvate dehydrogenase complex, see MIM 300502. The mammalian PDH complex differs from that in E. coli and from the other mammalian alpha-keto acid dehydrogenases by the presence of a 53-kD protein called protein X. Component X binds to the E3 (MIM 238331) component of the PDH complex (Robinson et al., 1990; Aral et al., 1997).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDHX Antibody (NB100-1496)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PDHX Antibody (NB100-1496) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PDHX
Entrez
Uniprot