PDHX Overexpression Lysate (Native)

Images

 
Western Blot: PDHX Overexpression Lysate (Adult Normal) [NBL1-14243] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PDHX.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PDHX Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203138 and based on accession number NM_003477. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens pyruvate dehydrogenase complex, component X (PDHX), nuclear gene encoding mitochondrial protein, transcript variant 1, mRNA.
Gene
PDHX

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
53.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for PDHX

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PDHX Overexpression Lysate (Native)

  • Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenasecomplex
  • DLDBP
  • E3-binding protein
  • E3BP
  • E3BP1
  • E3BPpyruvate dehydrogenase complex, E3-binding protein subunit
  • OPDX
  • PDHX
  • PDX1
  • PDX1Lipoyl-containing pyruvate dehydrogenase complex component X
  • proX
  • proXpyruvate dehydrogenase complex, lipoyl-containing component X
  • pyruvate dehydrogenase complex, component X
  • pyruvate dehydrogenase protein X component, mitochondrial

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
AF3444
Species: Hu
Applications: WB
NBP1-49672
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
MAB1249
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow
NBP1-31302
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-22218
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
AF2400
Species: Hu
Applications: WB, ChIP, ICC
NBP1-89522
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF2614
Species: Hu
Applications: WB, ICC
AF2746
Species: Hu, Mu
Applications: WB, ICC
NBP1-89150
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
H00011026-M01
Species: Hu
Applications: WB, ELISA
H00256297-M05
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB300-750
Species: Hu, Mu, Rt, Mk, Pm, Sh
Applications: WB, ICC/IF, IP
NBP2-27079
Species: Hu, Ca, Pm
Applications: WB, IHC-P
MAB2358
Species: Hu, Mu
Applications: IHC, ICC
NBP1-89680
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-47778
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-82554
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-88347
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for PDHX Lysate (NBL1-14243) (0)

There are no publications for PDHX Lysate (NBL1-14243).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PDHX Lysate (NBL1-14243) (0)

There are no reviews for PDHX Lysate (NBL1-14243). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PDHX Lysate (NBL1-14243) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional PDHX Products

Bioinformatics Tool for PDHX Lysate (NBL1-14243)

Discover related pathways, diseases and genes to PDHX Lysate (NBL1-14243). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PDHX Lysate (NBL1-14243)

Discover more about diseases related to PDHX Lysate (NBL1-14243).
 

Pathways for PDHX Lysate (NBL1-14243)

View related products by pathway.

PTMs for PDHX Lysate (NBL1-14243)

Learn more about PTMs related to PDHX Lysate (NBL1-14243).

Blogs on PDHX

There are no specific blogs for PDHX, but you can read our latest blog posts.

Customers Who Bought This Also Bought

NKX6.1 Antibody
NBP1-49672

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PDHX Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol PDHX
Entrez