PDGFRL Antibody (1008804) [Unconjugated]

A549 human lung carcinoma cell line was stained with Mouse anti-Human PDGFRL Monoclonal Antibody (Catalog # MAB1723, filled histogram) or isotype control antibody (MAB0041, open histogram) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 1008804
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PDGFRL Antibody (1008804) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived human PDGFRL
  Gln22-Ser375
  Accession # Q15198
• Specificity: Detects human PDGFRL in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGFRL Antibody (1008804) [Unconjugated]

• PDGFRL
• PDGFR-Like Protein
• PDGRL
• platelet-derived growth factor receptor-like protein
• platelet-derived growth factor receptor-like
• platelet-derived growth factor-beta-like tumor suppressor
• PRLTS
• PRLTSPDGF receptor beta-like tumor suppressor

Background

Platelet-derived growth factor receptor-like protein is a 67 kDa glycoprotein protein consisting of two Ig-like C2-type domains. By sequence similarity, human PDGFRL is 90% similar to the mouse version and 91% similar to that of the rat. It is a secreted protein related to the class III subfamily of receptor tyrosine kinases (RTK), the platelet-derived growth factor receptors (1-5). PDGFRL is a tumor suppressor active in the tumor-suppression network and implicated in colorectal cancer, and a decrease in PDGFRL expression levels has been observed in breast cancer (1). A variant of PDGFRL is found to play a role in the development of Behçet disease, a complex immunoregulatory disease (6). The autoimmune role of PDGFRL is also supported by its up-regulation in a mouse model for Rheumatoid Arthritis (7). Study also showed that PDGFRL may play a role in chondrocyte proliferation and differentiation.

1. Guo, F-J. et al. (2010) World J Gastroenterol. 16:1465.
2. Andrae, J. et al. (2008) Genes Dev. 22:1276.
3. Heldin, C-H. and B. Westermark (1999) Physiol. Rev. 79:1283.
4. Claesson-Welsh, L. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4917.
5. Matsui, T. et al. (1989) Science 243:800.
6. Hou, S. et al. (2013). Human Mutat. 34:74.
7. Fujikado, N. et al. (2006). Arthritis Res. & Ther. 8:R100.
8. Kawata, K. et al. (2017) J Cell Biochemistry. 118:4033.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q15198()