PDGFR alpha [p Tyr742] Antibody

Product Discontinued

PDGFR alpha [p Tyr742] Antibody Summary

• Immunogen: Synthetic phospho peptide that contains Tyrosine 742 (Human).
• Modification: p Tyr742
• Localization: Cell Membrane and Cytoplasmic.
• Specificity: PDGFR beta (50% identity) has not been tested, but is not expected to react.
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDGFRA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.35-1 ug/ml
• Application Notes: WB: Detects a band of approximately 170-185 kDa.

Reactivity Notes

Cross-reacts with Human and Mouse. Expected to cross-react with Rat (100% identity with immunogen), Xenopus laevis (Frog) (83% identity with immunogen) due to sequence identity, and PDGFRB is expected to react due to 50% sequence homology. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Dulbecco's PBS (pH 7.3) and 1.0 mg/ml
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDGFR alpha [p Tyr742] Antibody

• alpha-type platelet-derived growth factor receptor
• CD140 antigen-like family member A
• CD140a antigen
• CD140a
• EC 2.7.10
• EC 2.7.10.1
• MGC74795
• PDGF R alpha
• PDGFR alpha
• PDGFR2
• PDGFRA
• PDGFRA/BCR fusion
• PDGF-R-alpha
• platelet-derived growth factor receptor, alpha polypeptide
• rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein
• RHEPDGFRA

Background

Platelet derived growth factor receptor (PDGF Receptor) is a transmembrane glycoprotein of 170-185 kDa which undergoes homo or heterodimerization into complexes of alpha and beta subunits upon ligand binding, depending on the isoform of PDGF (PDGF AA, BB or AB) that binds. The phosphorylation of tyrosine residues in the now activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation, and also lead to activation of the Ras, Raf and ERK 1 and 2 pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGFR alpha Antibody (NSB1006)(3)

Publications using NSB1006

• Janssen ME, Liu H, Volkmann N, Hanein D. The C-terminal tail domain of metavinculin, vinculin's splice variant, severs actin filaments. J Cell Biol 2012-05-28 [PMID: 22613835] (Human)
• Hsieh CH, Chang HT, Shen WC, Shyu WC, Liu RS. Imaging the Impact of Nox4 in Cycling Hypoxia-mediated U87 Glioblastoma Invasion and Infiltration. Mol Imaging Biol. 2011-08-26 [PMID: 21870211] (WB, Human)
• Zheng L, Ishii Y, Tokunaga A et al. Neuroprotective effects of PDGF against oxidative stress and the signaling pathway involved. J Neurosci Res 2010-05-01 [PMID: 19998489] (WB, Mouse)

Bioinformatics

• Gene Symbol: PDGFRA
• Entrez:
  • 18595(Mouse)