PDGFR alpha Antibody (PRa292) [Allophycocyanin]

U-118-MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human PDGF Ra APC-conjugated Monoclonal Antibody (Catalog # FAB1264A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: PRa292
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

PDGFR alpha Antibody (PRa292) [Allophycocyanin] Summary

• Immunogen: Human osteosarcoma cell membrane extracts
• Specificity: Detects human PDGF R alpha . Recognizes the PDGF receptor alpha -subunit; it does not recognize the PDGF receptor beta -subunit. Binds to the PDGF receptor alpha -subunit of primate species (human, monkey, baboon) and dog. Does not recognize rat or mouse receptors and its ability to bind to receptors from other species has not been tested.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDGFRA
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGFR alpha Antibody (PRa292) [Allophycocyanin]

• alpha-type platelet-derived growth factor receptor
• CD140 antigen-like family member A
• CD140a antigen
• CD140a
• EC 2.7.10
• EC 2.7.10.1
• MGC74795
• PDGF R alpha
• PDGFR alpha
• PDGFR2
• PDGFRA
• PDGFRA/BCR fusion
• PDGF-R-alpha
• platelet-derived growth factor receptor, alpha polypeptide
• rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein
• RHEPDGFRA

Background

PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF‑BB and AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGF-R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).

1. Heldin, C.H. and L. Claesson-Welsh (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A. (ed) Oxford University Press, New York, NY p. 202.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGFR alpha Antibody (FAB1264A)(11)

Publications using FAB1264A

• Bisson, JA;Gordillo, M;Kumar, R;de Silva, N;Yang, E;Banks, KM;Shi, ZD;Lee, K;Yang, D;Chung, WK;Huangfu, D;Evans, T; GATA6 regulates WNT and BMP programs to pattern precardiac mesoderm during the earliest stages of human cardiogenesis bioRxiv : the preprint server for biology 2024-07-11 [PMID: 39026742] (Flow Cytometry, Human)
• Stanton, AE;Bubnys, A;Agbas, E;James, B;Park, DS;Jiang, A;Pinals, RL;Truong, N;Loon, A;Staab, C;Liu, L;Cerit, O;Wen, HL;Kellis, M;Blanchard, JW;Langer, RS;Tsai, LH; Engineered 3D Immuno-Glial-Neurovascular Human Brain Model bioRxiv : the preprint server for biology 2023-08-17 [PMID: 37645757] (Flow Cytometry, Human)
• S Marchiano, K Nakamura, H Reinecke, L Neidig, M Lai, S Kadota, F Perbellini, X Yang, JM Klaiman, LP Blakely, E Karbassi, PA Fields, AM Fenix, KM Beussman, A Jayabalu, FA Kalucki, A Futakuchi-, GJ Weber, S Dupras, H Tsuchida, L Pabon, L Wang, BC Knollmann, S Kattman, RS Thies, N Sniadecki, WR MacLellan, A Bertero, CE Murry Gene editing to prevent ventricular arrhythmias associated with cardiomyocyte cell therapy Cell Stem Cell, 2023-04-06;30(4):396-414.e9. 2023-04-06 [PMID: 37028405] (Flow Cytometry, Mouse)
• Y Lan, KM Banks, H Pan, N Verma, GR Dixon, T Zhou, B Ding, O Elemento, S Chen, D Huangfu, T Evans Stage-specific regulation of DNA methylation by TET enzymes during human cardiac differentiation Cell Reports, 2021-12-07;37(10):110095. 2021-12-07 [PMID: 34879277] (Flow Cytometry, ICC, Western Blot, Human)
• H Fukushima, M Yoshioka, M Kawatou, V López-Dávi, M Takeda, Y Kanda, Y Sekino, Y Yoshida, JK Yamashita Specific induction and long-term maintenance of high purity ventricular cardiomyocytes from human induced pluripotent stem cells PLoS ONE, 2020-11-02;15(11):e0241287. 2020-11-02 [PMID: 33137106] (MACS, Mouse)
• S Mount, P Kanda, S Parent, S Khan, C Michie, L Davila, V Chan, RA Davies, H Haddad, D Courtman, DJ Stewart, DR Davis Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium Stem Cell Res Ther, 2019-11-04;10(1):316. 2019-11-04 [PMID: 31685023] (Flow Cytometry, Human)
• Bertero A, Fields P A et al. Chromatin compartment dynamics in a haploinsufficient model of cardiac laminopathy. J Cell Biol 2/9/2019 [PMID: 31395619]
• NJ Palpant, Y Wang, B Hadland, RJ Zaunbreche, M Redd, D Jones, L Pabon, R Jain, J Epstein, WL Ruzzo, Y Zheng, I Bernstein, A Margolin, CE Murry Chromatin and Transcriptional Analysis of Mesoderm Progenitor Cells Identifies HOPX as a Regulator of Primitive Hematopoiesis Cell Rep, 2017-08-15;20(7):1597-1608. 2017-08-15 [PMID: 28813672] (Flow Cytometry, Human)
• Cloche is a bHLH-PAS transcription factor that drives haemato-vascular specification Nature, 2016-07-14;535(7611):294-8. 2016-07-14 [PMID: 27411634] (Flow Cytometry, Human)
• Vincent C. Chen, Jingjing Ye, Praveen Shukla, Giau Hua, Danlin Chen, Ziguang Lin et al. Development of a scalable suspension culture for cardiac differentiation from human pluripotent stem cells Stem Cell Research 9/1/2015 [PMID: 26318718]
• Craft A, Rockel J, Nartiss Y, Kandel R, Alman B, Keller G Generation of articular chondrocytes from human pluripotent stem cells. Nat Biotechnol, 2015-05-11;33(6):638-45. 2015-05-11 [PMID: 25961409] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: PDGFRA