PDGF R beta Antibody (4C12)

Western Blot: PDGF R beta Antibody (4C12) [H00005159-M08] - Analysis of PDGFRB expression in human uterus myoma.

Western Blot: PDGF R beta Antibody (4C12) [H00005159-M08] - Analysis of PDGFRB expression in human stomach.

ELISA: PDGF R beta Antibody (4C12) [H00005159-M08] - Detection limit for recombinant GST tagged PDGFRB is approximately 1ng/ml as a capture antibody.

Immunoprecipitation: PDGF R beta Antibody (4C12) [H00005159-M08] - Analysis of PDGFRB transfected lysate using anti-PDGFRB monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PDGFRB rabbit polyclonal antibody.

Proximity Ligation Assay: PDGF R beta Antibody (4C12) [H00005159-M08] - Analysis of protein-protein interactions between STAT1 and PDGFRB. Mahlavu cells were stained with anti-STAT1 rabbit purified polyclonal 1:1200 and anti-PDGFRB mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

PDGF R beta Antibody (4C12) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PDGFRB (AAH32224, 33 a.a. ~ 133 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LVVTPPGPELVLNVSSTFVLTCSGSAPVVWERMSQEPPQEMAKAQDGTFSSVLTLTNLTGLDTGEYFCTHNDSRGLETDERKRLYIFVPDPTVGFLPNDAE
• Specificity: PDGFRB (4C12)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDGFRB
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Proximity Ligation Assay
  • Western Blot 1:500
• Application Notes: Antibody reactivity against tissue lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PDGF R beta Antibody (4C12)

• beta-type platelet-derived growth factor receptor
• CD140 antigen-like family member B
• CD140b antigen
• CD140b
• EC 2.7.10
• EC 2.7.10.1
• JTK12
• PDGF R beta
• PDGFR
• PDGFR1
• PDGFRB
• PDGF-R-beta
• platelet-derived growth factor receptor, beta polypeptide

Background

This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDGFRB
• Entrez:
  • 5159(Human)
• OMIM:
  • 131440(Human)
• Uniprot:
  • AAH32224(Human)