PDGF-AA Antibody [Unconjugated]

Recombinant Human PDGF‑AA (Catalog # 221-AA) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF‑AA (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF‑AA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-221-NA). The ND50 is typically0.1-0.3 µg/mL.

PDGF-AA was detected in immersion fixed paraffin-embedded sections of human breast and breast cancer tissue using Goat Anti-Human PDGF-AA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-221-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PDGF-AA Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PDGF-AA
  Ser87-Thr211
  Accession # P04085
• Specificity: Detects human PDGF-AA and PDGF‑AB in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human PDGF-BB and recombinant rat PDGF-BB is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDGFA
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.1-0.3 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGF-AA Antibody [Unconjugated]

• PDGF1
• PDGF-A
• PDGFAA
• PDGF-AA

Background

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the
alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta . Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGF-AA Antibody (AF-221-NA)(7)

Publications using AF-221-NA

• Elias G. Elias, Joanne H. Hasskamp, Bhuvnesh K. Sharma Cytokines and Growth Factors Expressed by Human Cutaneous Melanoma Cancers (Basel) 5/7/2010 [PMID: 24281094]
• Wagsater D, Zhu C, Bjorck HM, Eriksson P Effects of PDGF-C and PDGF-D on monocyte migration and MMP-2 and MMP-9 expression. Atherosclerosis, 2008-05-22;202(2):415-23. 2008-05-22 [PMID: 18573494] (Neutralization, Human)
• Riggi N, Cironi L, Provero P, Suva ML, Stehle JC, Baumer K, Guillou L, Stamenkovic I Expression of the FUS-CHOP fusion protein in primary mesenchymal progenitor cells gives rise to a model of myxoid liposarcoma. Cancer Res., 2006-07-15;66(14):7016-23. 2006-07-15 [PMID: 16849546] (ICC, Mouse)
• Shikada Y, Yonemitsu Y, Koga T, Onimaru M, Nakano T, Okano S, Sata S, Nakagawa K, Yoshino I, Maehara Y, Sueishi K Platelet-derived growth factor-AA is an essential and autocrine regulator of vascular endothelial growth factor expression in non-small cell lung carcinomas. Cancer Res., 2005-08-15;65(16):7241-8. 2005-08-15 [PMID: 16103075] (IHC-P, Human)
• Helantera I, Loginov R, Koskinen P, Tornroth T, Gronhagen-Riska C, Lautenschlager I Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts. Nephrol. Dial. Transplant., 2005-02-16;20(4):790-6. 2005-02-16 [PMID: 15716293] (IHC, Human)
• Nagineni CN, Kutty V, Detrick B, Hooks JJ Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta. J. Cell. Physiol., 2005-04-01;203(1):35-43. 2005-04-01 [PMID: 15368539] (ICC, Human)
• Zhou W, Park I, Pins M, Kozlowski JM, Jovanovic B, Zhang J, Lee C, Ilio K Dual regulation of proliferation and growth arrest in prostatic stromal cells by transforming growth factor-beta1. Endocrinology, 2003-07-24;144(10):4280-4. 2003-07-24 [PMID: 12959966] (Neutralization, Human)

Bioinformatics

• Gene Symbol: PDGFA
• Uniprot:
  • P04085()