PDGF-AA Products

221-AA) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-AA (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-AA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-221-NA). The ND₅₀ is typically 0.1-0.3 µg/mL." class="big_lightbox" target="_blank">

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">

222-AB) was serially diluted and captured by Mouse Anti-Human PDGF‑B Monoclonal Antibody (Catalog # MAB1739) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human PDGF‑AA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-221-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

221-AA) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-AA (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-AA Polyclonal Antibody (Catalog # AB-221-NA). The ND₅₀ is typically 5-10 µg/mL." class="big_lightbox" target="_blank">

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">

1055-AA) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat PDGF-AA (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat PDGF-AA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1055). The ND₅₀ is typically 0.2-0.6 µg/mL." class="big_lightbox" target="_blank">

221-AA stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner. The ED₅₀ for this effect is 50-200 ng/mL." class="big_lightbox" target="_blank">

1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media Supplements (AR013 and AR003), and a panel of growth factors for effective oligodendrocyte differentiation, including Recombinant Human FGF-basic, Recombinant Human EGF (236-EG), and Recombinant Human PDGF-AA (Catalog # 221-AA). Oligodendrocytes were detected using a Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 Monoclonal Antibody (MAB1326). The cells were stained with the NorthernLights™-557 Affinity-purified Goat Anti-Mouse IgM Secondary Antibody (NL019; red). The nuclei were counterstained with DAPI (5748; blue)." class="big_lightbox" target="_blank">