PDE6D Antibody

Images

 
Western Blot: PDE6D Antibody [NBP1-32730] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [N1C3] diluted at 1:15000. The HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: PDE6D Antibody [NBP1-32730] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: PDE6D Antibody [NBP1-32730] - Paraffin-embedded SAS xenograft, using antibody at 1:500 dilution.
Western Blot: PDE6D Antibody [NBP1-32730] - Sample (30 ug of whole cell lysate) A: MOLT4 12% SDS PAGE diluted at 1:1000

Product Details

Summary
Reactivity Hu, Mu, Bv, Ca, RM, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PDE6D Antibody Summary

Immunogen
Full length human PDE6D Recombinant protein.
Predicted Species
Canine (98%), Bovine (99%), Zebrafish (90%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDE6D
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PDE6D Antibody

  • GMP-PDE delta
  • PDED
  • phosphodiesterase 6D, cGMP-specific, rod, delta
  • Protein p17
  • retinal rod rhodopsin-sensitive cGMP 3'-5'-cyclic phosphodiesterase subunitdelta

Background

Human PDE6D was originally identified as a fourth subunit of rod-specific cGMP phosphodiesterase, PDE6. Catalytically active PDE6 is a heterodimer (alpha beta) that is regulated by two inhibitory gamma subunits. As PDE6D does not modify the catalytic properties of PDE alpha beta, its function is still unresolved. Recombinant human PDE6D, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PDE6D