PDE6D Antibody (HL1359)

PDE6D antibody [HL1359] detects PDE6D protein at nucleus by immunofluorescent analysis.Sample: MOLT-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PDE6D stained by PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:500.Blue: Fluoroshield with DAPI .

PDE6D antibody [HL1359] detects PDE6D protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: PDE6D stained by PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:100.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

PDE6D antibody [HL1359] detects PDE6D protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat colon.PDE6D stained by PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole Japanese medaka extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

PDE6D antibody [HL1359] detects PDE6D protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.PDE6D stained by PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PDE6D antibody [HL1359] detects PDE6D protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.PDE6D stained by PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Ca, Fe, Ze
• Applications: WB, ICC/IF, IHC
• Clone: HL1359
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PDE6D Antibody (HL1359) Summary

• Description: Novus Biologicals Rabbit PDE6D Antibody (HL1359) (NBP3-48654) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Full length human PDE6D Recombinant protein
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PDE6D
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for PDE6D Antibody (HL1359)

• GMP-PDE delta
• PDE6D
• PDED
• phosphodiesterase 6D, cGMP-specific, rod, delta
• Protein p17
• retinal rod rhodopsin-sensitive cGMP 3'-5'-cyclic phosphodiesterase subunitdelta

Background

PDE6D is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system. The second messengers cAMP and cGMP are key regulatory molecules that are involved in a wide variety of signal transduction pathways, such as insulin secretion, platelet aggregation, smooth muscle relaxation, olfaction, and vision. Levels of cAMP and cGMP are regulated by their rate of synthesis by nucleotide cyclases and by their rate of hydrolysis by cyclic nucleotide phosphodiesterases (PDEs). PDEs form a superfamily of enzymes that catalyze the conversion of 3-prime, 5-prime-cyclic nucleotides to the corresponding nucleoside 5-prime-monophosphates. While mammalian PDEs are divided into major families based on their substrate specificities, kinetic properties, allosteric regulators, inhibitor sensitivities, and amino acid sequences, each family and even members within a family display distinct tissue, cell, and subcellular expression patters. This suggests that individual PDE family members are involved in discrete signal transduction pathways. There are five different subunits consisting of rod and cone specific catalytic subunits: alpha® (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family). The catalytic core of the PDE6 system is comprised of alpha®/alpha® homodimers in the cone and alpha/beta heterodimers in the rod. The C-terminus of both the catalytic and inhibitory subunits is modified by methylation, myristyolation and prenylation which have been shown to be critical for proper complex assembly and membrane association.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDE6D