PD-L2/B7-DC/PDCD1LG2 Antibody (24F.10C12) - BSA Free

Separation of human CD11c positive HLA-DR positive cells (red-filled) from CD11c negative HLA-DR negative cells (black-dashed) in Analysis (surface staining) of human stimulated (GM-CSF + IL-4) monocytes stained using anti-human CD273 (24F.10C12) purified antibody (concentration in sample 0.6 ug/ml) GAM APC.

• Reactivity: Hu
• Applications: Flow, IHC
• Clone: 24F.10C12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PD-L2/B7-DC/PDCD1LG2 Antibody (24F.10C12) - BSA Free Summary

• Immunogen: human PD-L2/B7-DC/PDCD1LG2
• Specificity: The mouse monoclonal antibody 24F.10C12 recognizes an extracellular epitope of CD273 / PD-L2 (also known as B7-DC), a 25 kDa type I transmembrane protein expressed by dendritic cells, activated monocytes and T cells, heart, first trimester placenta, lung and liver, as well as in Hodgkin's lymphoma cells and primary mediastinal B cell lymphoma (PMBL).
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDCD1LG2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-4 ug/ml
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PD-L2/B7-DC/PDCD1LG2 Antibody (24F.10C12) - BSA Free

• B7-DC
• bA574F11.2
• Btdc
• Butyrophilin B7-DC
• Butyrophilin-like Protein
• CD273 antigen
• CD273
• CD273PD-1 ligand 2
• MGC142240
• PD-1-ligand 2
• PDCD1L2MGC142238
• PDCD1LG2
• PDL2
• PD-L2
• PDL2B7DC
• PD-L2PDCD1 ligand 2
• programmed cell death 1 ligand 2
• Programmed death ligand 2

Background

B7-DC is also called programmed death ligand 2 (PDL2). It has recently been clustered as CD273. This ligand is a 42 kD member of the immunoglobulin receptor superfamily expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. B7-DC (CD273) has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. B7-DC (CD273) has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDCD1LG2