PD-L2/B7-DC/PDCD1LG2 Antibody (1054620) [Unconjugated]

In a functional flow cytometry test, Recombinant Human PD-1 Fc Chimera Alexa Fluor® 647 Protein (AFR1086) binds to HEK293 cells transfected with Human PD-L2 and eGFP. (A) Binding is completely blocked by 25 µg/mL of Mouse Anti-Human PD‑L2/B7‑DC Monoclonal Antibody (Catalog # MAB11351) but not by (B) Mouse IgG1 Isotype Control (Catalog # MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol.

• Reactivity: Hu
• Applications: B/N
• Clone: 1054620
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PD-L2/B7-DC/PDCD1LG2 Antibody (1054620) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived recombinant human PD-L2/B7-DC
  Accession # Q9BQ51
• Specificity: Detects human PD-L2/B7-DC in direct ELISA.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PD-L2/B7-DC/PDCD1LG2 Antibody (1054620) [Unconjugated]

• B7-DC
• bA574F11.2
• Btdc
• Butyrophilin B7-DC
• Butyrophilin-like Protein
• CD273 antigen
• CD273
• CD273PD-1 ligand 2
• MGC142240
• PD-1-ligand 2
• PDCD1L2MGC142238
• PDCD1LG2
• PDL2
• PD-L2
• PDL2B7DC
• PD-L2PDCD1 ligand 2
• programmed cell death 1 ligand 2
• Programmed death ligand 2

Background

Programmed Death Ligand 2 (PD-L2), also known as B7-DC and butyrophilin-like protein, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1). Mature human PD-L2 consists of a 201 amino acid (aa) extracellular domain (ECD) with one V-like and one C-like Ig domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2, 3). Within the ECD, mouse and human PD-L2 share 72% aa sequence identity. Alternative splicing generates additional isoforms that lack the second Ig-like domain and may be substituted and truncated following the first Ig-like domain (4). PD-L2 is expressed on dendritic cells, subsets of activated CD4⁺ and CD8⁺ T cells, and memory B cells that differentiate into plasma cells (3, 5, 6). At inflammatory sites such as rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is up-regulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells (7-11). PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 12, 13). In addition, PD-L2 binds to RGM-B on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (14). In asthma, PD-L2 suppresses IL-5 and IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (9, 11).

1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
2. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
3. Tseng, S.-Y. et al. (2001) J. Exp. Med. 193:839.
4. He, X.-H. et al. (2004) Acta Biochim. Biophys. Sinica 36:284.
5. Messal, N. et al. (2011) Mol. Immunol. 48:2214.
6. Zuccarino-Catania, G.V. et al. (2014) Nat. Immunol. 15:631.
7. Guo, G. et al. (2012) Clin. Rheumatol. 31:271.
8. Loke, P. and J.P. Allison (2003) Proc. Natl. Acad. Sci. USA 100:5336.
9. Matsumoto, K. et al. (2004) J. Immunol. 172:2530.
10. Stanciu, L.A. et al. (2006) J. Infec. Dis. 193:404.
11. Lewkowich, I.P. et al. (2013) Mucosal Immun. 6:728.
12. Ghiotto, M. et al. (2010) Int. Immunol. 22:651.
13. Shin, T. et al. (2003) J. Exp. Med. 198:31.
14. Xiao, Y. et al. (2014) J. Exp. Med. 211:943.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9BQ51()