PCNA Antibody

Western Blot: PCNA Antibody [NBP1-78105] - Western blot using affinity purified anti-PCNA antibody shows detection of PCNA antibody. Lane 1: HEK293 whole cell extracts. Lane 2: Jurkat whole cell extracts. Approximately 25ug of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose. After blocking, the membrane was incubated with the primary antibody diluted to 1:1000. The membrane was washed and reacted with a 1:10,000 dilution of IRDye(R) 800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10 (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel, red). Other detection systems will yield similar results.

N/A: PCNA Antibody [NBP1-78105]

Product Discontinued

PCNA Antibody Summary

• Description: This affinity purified antibody is directed against human PCNA protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified PCNA Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PCNA protein. (Uniprot: P12004)
• Marker: Proliferation Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PCNA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000-1:8000
  • Western Blot 1:500-1:2000
• Application Notes: This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 29-36 kDa in size corresponding to PCNA protein by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross reactivity with PCNA protein from human, monkey, dog, mouse, rat, bovine, chicken, fish and Xenopus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This affinity purified antibody is directed against human PCNA protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for PCNA Antibody

• cyclin
• DNA polymerase delta auxiliary protein
• MGC8367
• PCNA
• proliferating cell nuclear antigen

Background

The proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the processibility of DNA polymerase during elongation of the leading strand. PCNA is expressed in the nucleus of all proliferating cells and is pivotal for DNA synthesis and cell cycle progression. In response to DNA damage, PCNA is mono-ubiquitinated and is involved in mismatch-provoked excision. PCNA is a useful marker for DNA synthesis and is highly conserved among most species.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCNA
• Uniprot:
  • P12004(Human)