Human PCMT1 ELISA Kit (Colorimetric)

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ELISA: Human PCMT1 ELISA Kit (Colorimetric) [NBP3-39053] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
31.25 - 2000 pg/mL (example only; lot dependent)
Sensitivity
13.8 pg/mL (example only; lot dependent)

Order Details

Human PCMT1 ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
31.25 - 2000 pg/mL (example only; lot dependent)
Sensitivity
13.8 pg/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PCMT1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Reagent A
  2. Detection Reagent B
  3. Diluent Buffer
  4. Instruction manual
  5. Plate sealer for 96 wells
  6. Pre-coated 96T strip plate
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human PCMT1 ELISA Kit (Colorimetric)

  • EC 2.1.1
  • EC 2.1.1.77
  • L-isoaspartyl protein carboxyl methyltransferase
  • L-isoaspartyl/D-aspartyl methyltransferase
  • PIMT
  • Protein L-isoaspartyl/D-aspartyl methyltransferase
  • Protein-beta-aspartate methyltransferase
  • protein-L-isoaspartate (D-aspartate) O-methyltransferase
  • protein-L-isoaspartate(D-aspartate) O-methyltransferase

Background

Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for PCMT1 ELISA Kit (NBP3-39053). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol PCMT1