PCK2 Antibody

Images

 
Western Blot: PCK2 Antibody [NB100-2422] - Staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PCK2 Antibody Summary

Immunogen
Peptide with sequence KPWKPGDKEPCAH corresponding to internal region according to NP_004554.2.
Specificity
This antibody is expected to recognise isoform 1 (NP_004554) only, no cross-reactivity with PCK1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PCK2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Approx. 75 kDa band observed in human liver lysates is reported in scientific literature (PMID: 23466304.
Positive Control
Liver Lysate (NB820-59232)
PCK2 Lysate (NBL1-14180)
Publications
Read Publications using
NB100-2422 in the following applications:

  • WB
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine, Porcine and Mouse

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PCK2 Antibody

  • EC 4.1.1.32
  • PE
  • PEPCK
  • PEPCK2PEP carboxykinase
  • PEPCK-M
  • phosphoenolpyruvate carboxykinase [GTP], mitochondrial
  • phosphoenolpyruvate carboxykinase 2 (mitochondrial)
  • Phosphoenolpyruvate carboxylase
  • phosphopyruvate carboxylase

Background

This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PCK2 Antibody (NB100-2422)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PCK2
Entrez
Uniprot