PCIF1 Antibody - BSA Free

Western Blot: PCIF1 Antibody [NBP2-97683] - Anti-PCIF1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 81 kDa. Observed band size: 81 kDa.

Immunoprecipitation: PCIF1 Antibody [NBP2-97683] - PCIF1 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-PCIF1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PCIF1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 81 kDa. Observed band size: 81 kDa

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PCIF1 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human PCIF1 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PCIF1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for PCIF1 Antibody - BSA Free

• bA465L10.1
• C20orf67
• chromosome 20 open reading frame 67
• PDX-1 C terminus-interacting factor 1
• PDX1 C-terminal inhibiting factor 1
• phosphorylated CTD-interacting factor 1

Background

PCIF1 may play a role in transcription elongation or in coupling transcription to pre-mRNA processing through itsassociation with the phosphorylated C-terminal domain (CTD) ofRNAPIIlargest subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCIF1