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We offer PCIF1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each PCIF1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PCIF1 Antibodies can be used in a variety of model species: Human. Use the list below to choose the PCIF1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PCIF1 polyclonal antibodies.
![Immunohistochemistry: PCIF1 Antibody [NBP3-37957] - Immunohistochemistry analysis of paraffin-embedded Rat brain using PCIF1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37957_rabbit-pcif1-pab-1012202411421053.jpg "Immunohistochemistry: PCIF1 Antibody [NBP3-37957] - Immunohistochemistry analysis of paraffin-embedded Rat brain using PCIF1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.")
![Western Blot: PCIF1 Antibody [NBP3-37957] - Western blot analysis of various lysates using PCIF1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-37957_rabbit-pcif1-pab-1012202411343358.jpg)
![Immunohistochemistry: PCIF1 Antibody [NBP3-37957] - Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using PCIF1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37957_rabbit-pcif1-pab-1012202411392986.jpg)
![Immunoprecipitation: PCIF1 Antibody [NBP3-37957] - Immunoprecipitation analysis of 200 ug extracts of Jurkat cells using 3 ug PCIF1 antibody. Western blot was performed from the immunoprecipitate using PCIF1 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-37957_rabbit-pcif1-pab-101220241136017.jpg)
![Immunohistochemistry-Paraffin: PCIF1 Antibody [NBP2-13740] - Staining in human fallopian tube and liver tissues using anti-PCIF1 antibody. Corresponding PCIF1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PCIF1-Antibody-Immunohistochemistry-Paraffin-NBP2-13740-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PCIF1 Antibody [NBP2-13740] - Staining in human fallopian tube and liver tissues using anti-PCIF1 antibody. Corresponding PCIF1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PCIF1 Antibody [NBP2-13740] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/PCIF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13740-img0009.jpg)
![Immunohistochemistry-Paraffin: PCIF1 Antibody [NBP2-13740] - Staining of human fallopian tube shows high expression.](http://images.novusbio.com/fullsize/PCIF1-Antibody-Immunohistochemistry-Paraffin-NBP2-13740-img0006.jpg)
![Immunohistochemistry-Paraffin: PCIF1 Antibody [NBP2-13740] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/PCIF1-Antibody-Immunohistochemistry-Paraffin-NBP2-13740-img0007.jpg)
![Western Blot: PCIF1 Antibody - BSA Free [NBP3-47201] - Jurkat cells were subjected to SDS PAGE followed by western blot with (PCIF1 antibody) at dilution of 1:800](http://images.novusbio.com/fullsize/nbp3-47201_rabbit-pcif1-pab-bsa-free-28420251231269.jpg "Western Blot: PCIF1 Antibody - BSA Free [NBP3-47201] - Jurkat cells were subjected to SDS PAGE followed by western blot with (PCIF1 antibody) at dilution of 1:800")
![Immunohistochemistry: PCIF1 Antibody - BSA Free [NBP3-47201] - Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using (PCIF1 Antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-47201_rabbit-pcif1-pab-bsa-free-28420251221188.jpg)
![Western Blot: PCIF1 Antibody [H00063935-B01P] - Analysis of PCIF1 expression in transfected 293T cell line by PCIF1 polyclonal antibody. Lane 1: C20orf67 transfected lysate(77.44 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PCIF1-Antibody-Western-Blot-H00063935-B01P-img0002.jpg "Western Blot: PCIF1 Antibody [H00063935-B01P] - Analysis of PCIF1 expression in transfected 293T cell line by PCIF1 polyclonal antibody. Lane 1: C20orf67 transfected lysate(77.44 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PCIF1 Antibody [H00063935-B01P] - Analysis of purified antibody to C20orf67 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/PCIF1-Antibody-Immunocytochemistry-Immunofluorescence-H00063935-B01P-img0001.jpg)
![Immunoprecipitation:PCIF1 AntibodyNBP3-29865] -Detection of human PCIF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PCIF1 antibody NBP3-29865 (lot NBP3-29865-1) used for IP at 6 µg per reaction. PCIF1 was also immunoprecipitated by rabbit anti-PCIF1 antibody BL18025. For blotting immunoprecipitated PCIF1, NBP3-29865 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29865_rabbit-pcif1-pab-301220241735515.jpg "Immunoprecipitation:PCIF1 AntibodyNBP3-29865] -Detection of human PCIF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PCIF1 antibody NBP3-29865 (lot NBP3-29865-1) used for IP at 6 µg per reaction. PCIF1 was also immunoprecipitated by rabbit anti-PCIF1 antibody BL18025. For blotting immunoprecipitated PCIF1, NBP3-29865 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: PCIF1 Antibody [NBP2-97683] - Anti-PCIF1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 81 kDa. Observed band size: 81 kDa.](http://images.novusbio.com/fullsize/PCIF1-Antibody-Western-Blot-NBP2-97683-img0003.jpg "Western Blot: PCIF1 Antibody [NBP2-97683] - Anti-PCIF1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 81 kDa. Observed band size: 81 kDa.")
![Immunoprecipitation: PCIF1 Antibody [NBP2-97683] - PCIF1 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-PCIF1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PCIF1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 81 kDa. Observed band size: 81 kDa](http://images.novusbio.com/fullsize/PCIF1-Antibody-Immunoprecipitation-NBP2-97683-img0004.jpg)