Recombinant Human PCID2 His Protein

SDS-Page: PCID2 Recombinant Protein [NBP2-51816] - 15% SDS Page (3 ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human PCID2 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 80-399 of Human PCID2
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAYKCQTVIV QSFLRAFQAH KEENWALPVM YAVALDLRVF ANNADQQLVK KGKSKVGDML EKAAELLMSC FRVCASDTRA GIEDSKKWGM LFLVNQLFKI YFKINKLHLC KPLIRAIDSS NLKDDYSTAQ RVTYKYYVGR KAMFDSDFKQ AEEYLSFAFE HCHRSSQKNK RMILIYLLPV KMLLGHMPTV ELLKKYHLMQ FAEVTRAVSE GNLLLLHEAL AKHEAFFIRC GIFLILEKLK IITYRNLFKK VYLLLKTHQL SLDAFLVALK FMQVEDVDID EVQCILANLI YMGHVKGYIS HQHQKLVVSK QNPFPPLSTV C

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PCID2
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 39.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.4M UREA
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human PCID2 His Protein

• CSN12-like protein
• DKFZp686C20226
• F10
• FLJ11305
• FLJ99362
• MGC16774
• PCI domain containing 2
• PCI domain-containing protein 2

Background

PCID2, also known as PCI domain-containing protein 2, belongs to the CSN12 family, is a protein with 4 isoforms ranging from a 376 amino acid that is 43 kDa to a 453 amino acid that is 52 kDa, regulates the expression of cell-cycle checkpoint MAD2L1 protein during B cell differentiation necessary for B-cell survival. Disease research has shown correlation of this protein with malaria. This protein has been linked to the negative regulation of apoptotic process, regulation of mRNA stability, positive regulation of B cell differentiations, positive regulation of transcription, DNA-dependent and spleen development pathways where it interacts with KPNB1, SMAD2, BRF2, NEK6 and SHFM1 proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PCID2