PBX1 Antibody (PSH0-24)

Western Blot: PBX1 Antibody (PSH0-24) [NBP3-32720] - Western blot analysis of PBX1 on different lysates with Rabbit anti-PBX1 antibody (NBP3-32720) at 1/1,000 dilution. Lane 1: Hela cell lysate (20 ug/Lane) Lane 2: HEK-293 cell lysate (20 ug/Lane) Lane 3: MCF-7 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: Mouse testis tissue lysate (40 ug/Lane) Lane 8: Rat pancreas tissue lysate (40 ug/Lane) Predicted band size: 47 kDa Observed band size: 40/47 kDa Exposure time: 3 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32720) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PBX1 Antibody (PSH0-24) [NBP3-32720] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PBX1 antibody (NBP3-32720) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32720) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: PSH0-24
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PBX1 Antibody (PSH0-24) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human PBX1 aa 1-250 / 430. (Uniprot: P40424)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PBX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200
  • Western Blot 1:1000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PBX1 Antibody (PSH0-24)

• DKFZp686B09108
• Homeobox protein PBX1
• Homeobox protein PRL
• MGC126627
• Pre-B cell leukemia transcription factor-1
• pre-B-cell leukemia homeobox 1
• pre-B-cell leukemia transcription factor 1
• PRL

Background

PBX1 binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development a

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PBX1