PBX1 Antibody (OTI1C11) - Azide and BSA Free

Western Blot: PBX1 Antibody (OTI1C11) - Azide and BSA Free [NBP2-73265] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PBX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PBX1.

Immunocytochemistry/Immunofluorescence: PBX1 Antibody (OTI1C11) - Azide and BSA Free [NBP2-73265] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PBX1.

Immunohistochemistry: PBX1 Antibody (OTI1C11) - Azide and BSA Free [NBP2-73265] - Staining of paraffin-embedded Human liver tissue using anti-PBX1 mouse monoclonal antibody.

Flow Cytometry: PBX1 Antibody (OTI1C11) - Azide and BSA Free [NBP2-73265] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PBX1 antibody, and then analyzed by flow cytometry.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PBX1 Antibody (OTI1C11) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PBX1 Antibody (OTI1C11) - Azide and BSA Free (NBP2-00872) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PBX1(NP_002576) produced in HEK293 cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PBX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 46.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PBX1 Antibody (OTI1C11) - Azide and BSA Free

• DKFZp686B09108
• Homeobox protein PBX1
• Homeobox protein PRL
• MGC126627
• Pre-B cell leukemia transcription factor-1
• pre-B-cell leukemia homeobox 1
• pre-B-cell leukemia transcription factor 1
• PRL

Background

PBX1 binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development a

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PBX1