PBX1 Antibody (OTI1C11)

Western Blot: PBX1 Antibody (1C11) [NBP2-00872] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PBX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PBX1.

Immunocytochemistry/Immunofluorescence: PBX1 Antibody (1C11) [NBP2-00872] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PBX1.

Immunohistochemistry-Paraffin: PBX1 Antibody (1C11) [NBP2-00872] - Staining of paraffin-embedded Human liver tissue using anti-PBX1 mouse monoclonal antibody.

Flow Cytometry: PBX1 Antibody (1C11) [NBP2-00872] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PBX1 antibody, and then analyzed by flow cytometry.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.29 mg/ml

PBX1 Antibody (OTI1C11) Summary

• Description: Novus Biologicals Mouse PBX1 Antibody (OTI1C11) (NBP2-00872) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PBX1(NP_002576) produced in HEK293 cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PBX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 46.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.29 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PBX1 Antibody (OTI1C11)

• DKFZp686B09108
• Homeobox protein PBX1
• Homeobox protein PRL
• MGC126627
• Pre-B cell leukemia transcription factor-1
• pre-B-cell leukemia homeobox 1
• pre-B-cell leukemia transcription factor 1
• PRL

Background

This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis, and required for skeletal patterning and programming. A chromosomal translocation, t(1;19) involving this gene and TCF3/E2A gene, is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein, in which the DNA binding domain of E2A is replaced by the DNA binding domain of this protein, transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PBX1
• Entrez:
  • 5087(Human)