Parvalbumin Antibody

Western Blot: Parvalbumin Antibody [NB100-1541] - (0.01ug/ml) staining of Human Cerebellum (A) lysate + peptide (B) and (0.3ug/ml) Rat Brain (C) lysate + peptide (D) (35ug protein in RIPA buffer). Detected by chemiluminescence.

Flow Cytometry: Parvalbumin Antibody [NB100-1541] - Analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Parvalbumin Antibody Summary

• Immunogen: Peptide with sequence C-GVDEFSTLVAES corresponding to C-Terminus according to NP_002845.1.
• Epitope: C Terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PVALB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.01-0.3 ug/ml
• Application Notes: Western blot: Approx 15kDa band observed in Human Cerebellum lysates and approx 16kDa in Rat Brain lysates (calculated MW of 12.1kDa according to Human NP_002845.1 and 11.9kDa according to Rat NP_071944.1). These molecular weights are observed by other sources and were successfully blocked by incubation with the immunizing peptide.
  Flow Cytometry: Flow cytometric analysis of Kelly cells.
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Parvalbumin Antibody

• D22S749
• MGC116759
• parvalbumin alpha
• parvalbumin
• PV

Background

The protein encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Parvalbumin Antibody (NB100-1541)(2)

Publications using NB100-1541

• Xiong H, Xia K, Li B et al. KChIP1: a potential modulator to GABAergic system. gmp013. 2009-01-01 [PMID: 19352544]
• Hashimoto T, Bergen SE, Nguyen QL, Xu B, Monteggia LM, Pierri JN, Sun Z, Sampson AR, Lewis DA. Relationship of brain-derived neurotrophic factor its receptor TrkB to altered inhibitory prefrontal circuitry in schizophrenia. J Neurosci;25(2):372-83. 2005-01-12 [PMID: 15647480]

FAQs for Parvalbumin Antibody (NB100-1541). (Showing 1 - 1 of 1 FAQs).

1. I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?
   • We currently have three alpha7 nicotinic cholingergic receptor antibodies available for purchase. Please see this list for our Parvalbumin antibodies. None are currently validated in mouse. I would like to introduce you to our Innovators Reward Program. We have an excelent calbinin D28K antibody that has been validated for use in human and mouse and WB. The pricing and availability of our products depends on your country.

Bioinformatics

• Gene Symbol: PVALB
• Entrez:
  • 5816(Human)
  • 25269(Rat)
• Uniprot:
  • NP_002845.1(Human)