PARP2 Antibody (4G8)

Western Blot: PARP2 Antibody (4G8) [NBP2-89041] - PARP-2, mAb (4G8) (1:50) on Hela cells (7.5x105).

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 4G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PARP2 Antibody (4G8) Summary

• Immunogen: Recombinant mouse PARP2 (poly(ADP-ribose) polymerase-2).
• Specificity: Recognizes an epitope in domain E of PARP2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PARP2
• Purity: N/A

Applications/Dilutions

• Dilutions:
  • ELISA 1:500
  • Western Blot 1:20-1:50
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tissue culture supernatant
• Preservative: 0.1% Sodium Azide
• Purity: N/A

Alternate Names for PARP2 Antibody (4G8)

• ADP-ribosyltransferase (NAD+; poly(ADP-ribose) polymerase)-like 2
• ADPRT2
• ADPRT-2
• ADPRT2pADPRT-2
• ADPRTL2
• ADPRTL2EC 2.4.2.30
• ADPRTL3
• ARTD2
• EC 2.4.2.30
• hPARP-2
• NAD(+) ADP-ribosyltransferase 2
• pADPRT-2
• PARP2
• PARP-2
• poly (ADP-ribose) polymerase 2
• poly (ADP-ribose) polymerase family, member 2
• poly (ADP-ribosyl) transferase-like 2
• poly [ADP-ribose] polymerase 2
• poly(ADP-ribose) synthetase
• Poly[ADP-ribose] synthase 2
• poly[ADP-ribose] synthetase 2

Background

PARP2 encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PARP2