PARG Antibody - Azide and BSA Free

Western Blot: PARG Antibody [H00008505-B01P] - Analysis of PARG expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: PARG Antibody [H00008505-B01P] - Analysis of purified antibody to PARG on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: PARG Antibody [H00008505-B01P] - Expression in transfected 293T cell line. Lane 1: PARG transfected lysate(107.36 KDa). Lane 2: Non-transfected lysate.

Western Blot: PARG Antibody [H00008505-B01P] - Analysis of PARG expression in transfected 293T cell line by PARG polyclonal antibody. Lane 1: PARG transfected lysate(107.36 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu, Pl
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PARG Antibody - Azide and BSA Free Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: PARG (NP_003622.2, 1 a.a. - 976 a.a.) full-length human protein. MNAGPGCEPCTKRPRWGAATTSPAASDARSFPSRQRRVLDPKDAHVQFRVPPSSPACVPGRAGQHRGSATSLVFKQKTITSWMDTKGIKTAESESLDSKENNNTRIESMMSSVQKDNFYQHNVEKLENVSQLSLDKSPTEKSTQYLNQHQTAAMCKWQNEGKHTEQLLESEPQTVTLVPEQFSNANIDRSPQNDDHSDTDSEENRDNQQFLTTVKLANAKQTTEDEQAREAKSHQKCSKSCDPGEDCASCQQDEIDVVPESPLSDVGSEDVGTGPKNDNKLTRQESCLGNSPPFEKESEPESPMDVDNSKNSCQDSEADEETSPGFDEQEDGSSSQTANKPSRFQARDADIEFRKRYSTKGGEVRLHFQFEGGESRTGMNDLNAKLPGNISSLNVECRNSKQHGKKDSKITDHFMRLPKAEDRRKEQWETKHQRTERKIPKYVPPHLSPDKKWLGTPIEEMRRMPRCGIRLPLLRPSANHTVTIRVDLLRAGEVPKPFPTHYKDLWDNKHVKMPCSEQNLYPVEDENGERTAGSRWELIQTALLNKFTRPQNLKDAILKYNVAYSKKWDFTALIDFWDKVLEEAEAQHLYQSILPDMVKIALCLPNICTQPIPLLKQKMNHSITMSQEQIASLLANAFFCTFPRRNAKMKSEYSSYPDINFNRLFEGRSSRKPEKLKTLFCYFRRVTEKKPTGLVTFTRQSLEDFPEWERCEKPLTRLHVTYEGTIEENGQGMLQVDFANRFVGGGVTSAGLVQEEIRFLINPELIISRLFTEVLDHNECLIITGTEQYSEYTGYAETYRWSRSHEDGSERDDWQRRCTEIVAIDALHFRRYLDQFVPEKMRRELNKAYCGFLRPGVSSENLSAVATGNWGCGAFGGDARLKALIQILAAAAAERDVVYFTFGDSELMRDIYSMHIFLTERKLTVGDVYKLLLRYYNEECRNCSTPGPDIKLYPFIYHAVESCAETADHSGQRTGT
• Specificity: Reacts with poly (ADP-ribose) glycohydrolase.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: PARG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This antibody is reactive against transfected lysate in WB, IF and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PARG Antibody - Azide and BSA Free

• EC 3.2.1.143
• FLJ54459
• FLJ60257
• FLJ60456
• PARG
• PARG99
• poly (ADP-ribose) glycohydrolase
• poly(ADP-ribose) glycohydrolase 60 kDa isoform
• poly(ADP-ribose) glycohydrolase

Background

Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PARG Antibody (H00008505-B01P)(2)

Publications using H00008505-B01P

• Boudra MT, Bolin C, Chiker S et al. PARP-2 depletion results in lower radiation cell survival but cell line-specific differences in poly(ADP-ribose) levels. Cell Mol Life Sci. 2014-10-22 [PMID: 25336152]
• Arena C, Mistretta C, Di Natale E et al. Characterization and role of poly(ADP-ribosyl)ation in the Mediterranean species Cistus incanus L. under different temperature conditions. Plant Physiology et Biochemistry. 2011-01-01 [PMID: 21356593]

Bioinformatics

• Gene Symbol: PARG
• Entrez:
  • 8505(Human)
• Uniprot:
  • NP_003622.2(Human)