PARC Antibody

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Western Blot: PARC Antibody [NB100-2300] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit ...read more
Western Blot: PARC Antibody [NB100-2300] - Detection of Human PARC/H7-AP1 on HeLa whole cell lysate using NB100-2300. Normal rabbit IgG was used as a control for immunoprecipitation (IP Control).
Western Blot: PARC Antibody [NB100-2300] - Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARC/H7-AP1 antibody used for WB at 0.1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
     

Genetic Strategies

   

Order Details

PARC Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 900 and 950 of human P53-associated parkin-like cytoplasmic protein using the numbering given in TrEMBL entry Q8IWT3 (GeneID 23113).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CUL9
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunoprecipitation 2-5 ug/mg lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PARC Antibody

  • CUL-9
  • cullin 9
  • cullin-9
  • DKFZp686G1042
  • H7AP1DKFZp686P2024
  • p53-associated parkin-like cytoplasmic protein
  • PARCKIAA0708
  • parkin-like cytoplasmic p53 binding protein
  • RP3-330M21.2
  • UbcH7-associated protein 1

Background

PARC is a cytoplasmic anchor protein in p53 associated protein complexes. PARC directly interacted and formed an approximately 1MD complex with p53 in the cytoplasm of unstressed cells. In the absence of stress, inactivation of PARC induced nuclear localization of endogenous p53 and activated p53 dependent apoptosis. Overexpression of PARC promoted cytoplasmic sequestration of ectopic p53. Furthermore, abnormal cytoplasmic localization of p53 was observed in a number of neuroblastoma cell lines; RNA interference-mediated reduction of endogenous PARC significantly sensitized these neuroblastoma cells in the DNA damage response. These results revealed that PARC is a critical regulator in controlling p53 subcellular localization and subsequent function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CUL9
Entrez