PAR4 Antibody (5F4) - Azide and BSA Free

ELISA: PAR4 Antibody (5F4) [H00009002-M01] - Detection limit for recombinant GST tagged F2RL3 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Flow
• Clone: 5F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PAR4 Antibody (5F4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: F2RL3 (NP_003941, 18 a.a. ~ 82 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GGTQTPSVYDESGSTGGGDDSTPSILPAPRGYPGQVCANDSDTLELPDSSRALLLGWVPTRLVPA
• Localization: Integral Memebrane Protein
• Specificity: F2RL3 - coagulation factor II (thrombin) receptor-like 3
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: F2RL3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PAR4 Antibody (5F4) - Azide and BSA Free

• proteinase-activated receptor 4
• coagulation factor II (thrombin) receptor-like 3
• F2RL3
• PAR4
• Protease-Activated Receptor 4
• Thrombin Receptor-like 3

Background

Coagulation factor II (thrombin) receptor-like 3 (F2RL3) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL3 is also a member of the protease-activated receptor family. F2RL3 is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. F2RL3 is activated by thrombin and trypsin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PAR4 Antibody (H00009002-M01)(2)

Publications using H00009002-M01

• Sanchez Centellas D, Gudlur S, Vicente-Carrillo A et al. A cluster of aspartic residues in the extracellular loop II of PAR 4 is important for thrombin interaction and activation of platelets. Thromb Res 2017-04-13 [PMID: 28448853]
• Muehlschlegel JD, Perry TE, Liu KY et al. Polymorphism in the protease activated receptor-4 gene region associates with platelet activation and perioperative myocardial injury. American Journal of Hematology. 2012-01-01 [PMID: 22228373]

Bioinformatics

• Gene Symbol: F2RL3
• Entrez:
  • 9002(Human)
• OMIM:
  • 602779(Human)
• Uniprot:
  • NP_003941(Human)