PAR1/Thrombin Receptor Antibody (731115) [Phycoerythrin]

Human peripheral blood platelets were stained with Mouse Anti-Human Integrin a2b/CD41 APC-conjugated Monoclonal Antibody (Catalog # FAB7616A) and either (A) Mouse Anti-Human PAR1 PE-conjugated Monoclonal Antibody (Catalog # FAB3855P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

HT‑29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB3855P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 731115
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

PAR1/Thrombin Receptor Antibody (731115) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human PAR1
  Arg27-Thr102, Ser375-Thr425
  Accession # P25116
• Specificity: Detects human PAR1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: F2R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PAR1/Thrombin Receptor Antibody (731115) [Phycoerythrin]

• Cf2r
• CF2RHTR
• coagulation factor II (thrombin) receptor
• Coagulation factor II receptor
• F2R
• HTR
• PAR1
• PAR1PAR-1protease-activated receptor 1
• Protease-Activated Receptor 1
• Thrombin receptor
• ThrR
• TRGPC
• TRproteinase-activated receptor 1

Background

Human Proteinase-Activated Receptor 1 (hPAR1), also known as thrombin receptor, is a 65-70 kDa, 399 amino acid long member of the seven-transmembrane superfamily of cell-surface G protein-coupled receptors. PAR1 is activated by thrombin cleavage of its N-terminal propeptide in the extracellular domain. Human PAR1 is widely expressed in many cell types including endothelial cells, and it has been implicated in a variety of inflammatory responses. Over the regions used as immunogen, human and mouse PAR1 proteins are 58% identical in the region spanning the propeptide and extracellular domains, and 84% identical in the cytoplasmic tail.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PAR1/Thrombin Receptor Antibody (FAB3855P)(1)

Publication using FAB3855P

• Balenga N, Klichinsky M, Xie Z, Chan E, Zhao M, Jude J, Laviolette M, Panettieri R, Druey K A fungal protease allergen provokes airway hyper-responsiveness in asthma. Nat Commun, 2015-04-13;6(0):6763. 2015-04-13 [PMID: 25865874] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: F2R
• Uniprot:
  • P25116()