PAR1/Thrombin Receptor Antibody (731115) [Alexa Fluor® 488]

HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB3855G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Human peripheral blood platelets were stained with Mouse Anti-Human Integrin a2b/CD41 APC-conjugated Monoclonal Antibody (Catalog # FAB7616A) and either (A) Mouse Anti-Human PAR1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB3855G) or (B) Mouse IgG2BAlexa Fluor 488 Isotype Control (Catalog # IC0041G). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 731115
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

PAR1/Thrombin Receptor Antibody (731115) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human PAR1
  Arg27-Thr102, Ser375-Thr425
  Accession # P25116
• Specificity: Detects human PAR1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: F2R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PAR1/Thrombin Receptor Antibody (731115) [Alexa Fluor® 488]

• Cf2r
• CF2RHTR
• coagulation factor II (thrombin) receptor
• Coagulation factor II receptor
• F2R
• HTR
• PAR1
• PAR1PAR-1protease-activated receptor 1
• Protease-Activated Receptor 1
• Thrombin receptor
• ThrR
• TRGPC
• TRproteinase-activated receptor 1

Background

Human Proteinase-Activated Receptor 1 (hPAR1), also known as thrombin receptor, is a 65-70 kDa, 399 amino acid long member of the seven-transmembrane superfamily of cell-surface G protein-coupled receptors. PAR1 is activated by thrombin cleavage of its N-terminal propeptide in the extracellular domain. Human PAR1 is widely expressed in many cell types including endothelial cells, and it has been implicated in a variety of inflammatory responses. Over the regions used as immunogen, human and mouse PAR1 proteins are 58% identical in the region spanning the propeptide and extracellular domains, and 84% identical in the cytoplasmic tail.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PAR1/Thrombin Receptor Antibody (FAB3855G)(5)

Publications using FAB3855G

• Friebel, J;Wegner, M;Blöbaum, L;Schencke, PA;Jakobs, K;Puccini, M;Ghanbari, E;Lammel, S;Thevathasan, T;Moos, V;Witkowski, M;Landmesser, U;Rauch-Kröhnert, U; Characterization of Biomarkers of Thrombo-Inflammation in Patients with First-Diagnosed Atrial Fibrillation International journal of molecular sciences 2024-04-08 [PMID: 38612918] (Flow Cytometry, Human)
• Y Roka-Moiia, KR Ammann, S Miller-Gut, J Sheriff, D Bluestein, JE Italiano, RC Flaumenhaf, MJ Slepian Shear-Mediated Platelet Microparticles Demonstrate Phenotypic Heterogeneity as to Morphology, Receptor Distribution, and Hemostatic Function International Journal of Molecular Sciences, 2023-04-17;24(8):. 2023-04-17 [PMID: 37108551] (Flow Cytometry, Human)
• J Friebel, M Witkowski, M Wegner, L Blöbaum, S Lammel, PA Schencke, K Jakobs, M Puccini, D Rei beta ner, D Steffens, V Moos, HP Schutheiss, U Landmesser, U Rauch Cytotoxic CD8+ T Cells Are Involved in the Thrombo-Inflammatory Response during First-Diagnosed Atrial Fibrillation Cells, 2022-12-29;12(1):. 2022-12-29 [PMID: 36611934] (Flow Cytometry, Human)
• M Avril, M Benjamin, MM Dols, JD Smith Interplay of Plasmodium falciparum and thrombin in brain endothelial barrier disruption Sci Rep, 2019-09-11;9(1):13142. 2019-09-11 [PMID: 31511575] (Flow Cytometry, Human)
• Alejandro Alvarez-Ar, Irene Lee-River, Edith López, Arturo Hernández, Ana María López-Col Thrombin-Induced Calpain Activation Promotes Protease-Activated Receptor 1 Internalization. International Journal of Cell Biology, 2017-11-09;0(0):1687-8876. 2017-11-09 [PMID: 29250115] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: F2R
• Uniprot:
  • P25116()