PAR1/Thrombin Receptor Antibody (731115) [Unconjugated]

Western blot shows lysates of Y-79 human retinoblastoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PAR1 Monoclonal Antibody (Catalog # MAB3855) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PAR1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HT‑29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR1 Monoclonal Antibody (Catalog # MAB3855, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Human peripheral blood platelets were stained with Mouse Anti-Human Integrin alpha 2b/CD41 PE‑conjugated Monoclonal Antibody (Catalog # FAB7616P) and either (A) Mouse Anti-Human PAR1 Monoclonal Antibody (Catalog # MAB3855) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

PAR1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human PAR1 Monoclonal Antibody (Catalog # MAB3855) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: 731115
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PAR1/Thrombin Receptor Antibody (731115) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PAR1
  Arg27-Thr102, Ser375-Thr425
  Accession # P25116
• Specificity: Detects human PAR1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: F2R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PAR1/Thrombin Receptor Antibody (731115) [Unconjugated]

• Cf2r
• CF2RHTR
• coagulation factor II (thrombin) receptor
• Coagulation factor II receptor
• F2R
• HTR
• PAR1
• PAR1PAR-1protease-activated receptor 1
• Protease-Activated Receptor 1
• Thrombin receptor
• ThrR
• TRGPC
• TRproteinase-activated receptor 1

Background

Human Proteinase-Activated Receptor 1 (hPAR1), also known as thrombin receptor, is a 65-70 kDa, 399 amino acid long member of the seven-transmembrane superfamily of cell-surface G protein-coupled receptors. PAR1 is activated by thrombin cleavage of its N-terminal propeptide in the extracellular domain. Human PAR1 is widely expressed in many cell types including endothelial cells, and it has been implicated in a variety of inflammatory responses. Over the regions used as immunogen, human and mouse PAR1 proteins are 58% identical in the region spanning the propeptide and extracellular domains, and 84% identical in the cytoplasmic tail.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PAR1/Thrombin Receptor Antibody (MAB3855)(1)

Publication using MAB3855

• P Altieri, M Bertolotto, P Fabbi, E Sportelli, M Balbi, F Santini, C Brunelli, M Canepa, F Montecucco, P Ameri Data regarding the effects of thrombin and dabigatran-inhibited thrombin on protease-activated receptor 1 and activation of human atrial fibroblasts Data Brief, 2018-05-25;19(0):925-931. 2018-05-25 [PMID: 29900391] (ICC, Human)

Bioinformatics

• Gene Symbol: F2R
• Uniprot:
  • P25116()