pan Actin Antibody (AC-40)

Western Blot: pan Actin Antibody (AC-40) [NB120-11003] - Cell line lysates lanes: 1. HeLa, 2. JURKAT, 3. COS7, 4. NIH-3T3, 5. PC-12, 6. RAT2, 7. CHO, 8. MDBK, 9. MDCK.

Immunocytochemistry/Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003] - CFB cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with Monoclonal Anti-Actin produced in mouse clone No. AC-40. The antibody was developed using Anti-Mouse IgG (Fab specific)-FITC antibody produced in goat. Cells were counterstained with DAPI (blue) to stain nuclei.

Immunocytochemistry/Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003] - conserved C-terminus specific Monoclonal staining of human tongue frozen section with Monoclonal Anti-Actin

Immunocytochemistry/Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003] - Rat2 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:250 Monoclonal Anti-Actin Clone: AC-40. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

• Reactivity: Hu, Mu, Rt, Po, Am, Bv, Ca, Ch, ChHa, Gp, Gt, Pm, Rb, Re, Sh
• Applications: WB, Simple Western, ELISA, ICC/IF, IHC
• Clone: AC-40
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

pan Actin Antibody (AC-40) Summary

• Description: Novus Biologicals Mouse pan Actin Antibody (AC-40) (NB120-11003) is a monoclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and Simple Western. Anti-pan Actin Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide: SGPSIVHALCP (human) attached to Multiple Antigen Peptide (MAP) backbone.
• Epitope: Monoclonal anti-Actin recognizes an epitope located on the C-terminal end of actin. This epitope is conserved in all actin isoforms.
• Localization: Cytoplasmic
• Specificity: The does not react with the epitope on the N-terminal end of actin.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTA1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Simple Western
  • Western Blot 1:500
• Application Notes: For IHC: The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel's or Bouin's solutions may also be used as fixatives.
  See Simple Western Antibody Database for Simple Western validation: tested in HEK293 and mouse brain; separated by Size-Jess/Wes, Sally Sue/Peggy Sue, antibody dilution of 1:200; apparent MW in kDa on Simple Western was 50 kDa; matrix was 12-230 kDa.
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for pan Actin Antibody (AC-40)

• ACTA
• actin, alpha 1, skeletal muscle
• alpha skeletal muscle actin
• alpha skeletal muscle
• alpha-actin-1
• ASMA
• CFTD
• CFTDM
• MPFD
• NEM1
• NEM2
• NEM3

Background

Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for pan Actin Antibody (NB120-11003)(2)

Publications using NB120-11003

• Osorio FG, Soria-Valles C, Santiago-Fernandez O et al. Loss of the proteostasis factor AIRAPL causes myeloid transformation by deregulating IGF-1 signaling. Nat. Med. 2016-01-01 [PMID: 26692333] (WB, Mouse, Human)
• Bibeau K, Battista MC, Houde V et al. Fetal adrenal gland alterations in a rat model of adverse intrauterine environment. Am J Physiol Regul Integr Comp Physiol. 2009-11-18 [PMID: 19923365]

Review for pan Actin Antibody (NB120-11003) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Ranveer Singh WB Human 05/22/2014

Summary

• Application: Western Blot
• Sample Tested: siRNA transfected whole cell lysate from siRNA transfected HEK 293T cells
• Species: Human

Bioinformatics

• Gene Symbol: ACTA1
• Entrez:
  • 58(Human)
  • 60(Human)
  • 71(Human)
  • 11465(Mouse)
  • 287876(Rat)