PAK2 Antibody (6D12) - BSA Free

Immunoprecipitation: PAK2 Antibody (6D12) [NBP3-26442] - Immunoprecipitating PAK2 in Raji whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26442 in Raji whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26442 (2ug) + Raji whole cell lysate (500ug). Lane 3: Raji whole cell lysate (10ug).

Western Blot: PAK2 Antibody (6D12) [NBP3-26442] - Positive Western Blot detected in: 293 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, Mouse brain tissue, Rat brain tissue. All lanes: PAK2 Antibody at 1:2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 59 kDa Observed band size: 59 kDa

Flow Cytometry: PAK2 Antibody (6D12) [NBP3-26442] - Overlay histogram showing HepG2 cells stained with PAK2 Antibody (6D12) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, Flow, IP
• Clone: 6D12
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PAK2 Antibody (6D12) - BSA Free Summary

• Description: Novus Biologicals Rabbit PAK2 Antibody (6D12) - BSA Free (NBP3-26442) is a recombinant monoclonal antibody validated for use in WB, ELISA, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human PAK2 [UniProt Q13177]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PAK2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:20-1:200
  • Immunoprecipitation 1:200-1:1000
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PAK2 Antibody (6D12) - BSA Free

• EC 2.7.11
• gamma-PAK
• p21 (CDKN1A)-activated kinase 2
• p21 protein (Cdc42/Rac)-activated kinase 2
• p21-activated kinase 2
• p58
• PAK2
• PAK-2
• PAK65
• PAK65EC 2.7.11.1
• PAKgamma
• S6/H4 Kinase
• serine/threonine-protein kinase PAK 2

Background

p21-activated kinase 2 (PAK2) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). All six PAKs play an important role in diverse cellular processes, including cytoskeletal dynamics, growth/apoptotic signal transduction through MAP kinases, and regulation of transcription factors (4). The ubiquitous PAK-2 is unique among the PAK isoforms because it is also activated through proteolytic cleavage by caspases or caspase-like proteases (5). Full-length PAK-2 is localized in the cytoplasm, whereas the proteolytic PAK-2p34 fragment translocates to the nucleus. Activation of full-length PAK-2 stimulates cell survival, whereas proteolytic activation of PAK-2p34 is involved in programmed cell death (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAK2