PAK2 Antibody (3B5)

Western Blot: PAK2 Antibody (3B5) [NBP1-47542] - Western blot analysis using anti-PAK2 monoclonal antibody against Hela (1), Jurkat (2), A549 (3), HEK293 (4) and K562 (5) cell lysate.

Immunocytochemistry/Immunofluorescence: PAK2 Antibody (3B5) [NBP1-47542] - Confocal immunofluorescence analysis of Hela cells using anti-PAK2 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry-Paraffin: PAK2 Antibody (3B5) [NBP1-47542] - Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and gastric cancer (right) using anti-PAK2 antibody with DAB staining.

• Reactivity: Hu, Pm
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PAK2 Antibody (3B5) Summary

• Description: Novus Biologicals Mouse PAK2 Antibody (3B5) (NBP1-47542) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of PAK2 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAK2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1: 500-1:2000
• Application Notes: This antibody is useful in Western Blot, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunofluorescence, ELISA.
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for PAK2 Antibody (3B5)

• EC 2.7.11
• gamma-PAK
• p21 (CDKN1A)-activated kinase 2
• p21 protein (Cdc42/Rac)-activated kinase 2
• p21-activated kinase 2
• p58
• PAK2
• PAK-2
• PAK65
• PAK65EC 2.7.11.1
• PAKgamma
• S6/H4 Kinase
• serine/threonine-protein kinase PAK 2

Background

The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAK2