PAK2 Antibody (3B5)

Images

 
Western Blot: PAK2 Antibody (3B5) [NBP1-47542] - Western blot analysis using anti-PAK2 monoclonal antibody against Hela (1), Jurkat (2), A549 (3), HEK293 (4) and K562 (5) cell lysate.
Immunocytochemistry/ Immunofluorescence: PAK2 Antibody (3B5) [NBP1-47542] - Confocal immunofluorescence analysis of Hela cells using anti-PAK2 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: PAK2 Antibody (3B5) [NBP1-47542] - Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and gastric cancer (right) using anti-PAK2 antibody with DAB staining.

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
3B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PAK2 Antibody (3B5) Summary

Immunogen
Purified recombinant fragment of PAK2 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PAK2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1: 500-1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This antibody is useful in Western Blot, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunofluorescence, ELISA.
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for PAK2 Antibody (3B5)

  • EC 2.7.11
  • gamma-PAK
  • p21 (CDKN1A)-activated kinase 2
  • p21 protein (Cdc42/Rac)-activated kinase 2
  • p21-activated kinase 2
  • p58
  • PAK2
  • PAK-2
  • PAK65
  • PAK65EC 2.7.11.1
  • PAKgamma
  • S6/H4 Kinase
  • serine/threonine-protein kinase PAK 2

Background

The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PAK2
Entrez