PAG3 293T Cell Transient Overexpression Lysate

DDEF2 transfected lysate.

Lane 1: DDEF2 transfected lysate (110.77 KDa) Lane 2: Non-transfected lysate.

Product Discontinued

PAG3 293T Cell Transient Overexpression Lysate Summary

• Description: Quality control test: Transient overexpression cell lysate was tested with Anti-DDEF2 antibody by Western Blots. Plasmid: pCMV-DDEF2 full-length
• Specificity: DDEF2 293T Cell Transient Overexpression Lysate(Denatured)
• Gene: ASAP2

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

• Type: 293T Cell Transient Overexpression
• Protein State: Denatured

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PAG3 293T Cell Transient Overexpression Lysate

• arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2
• ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
• centaurin, beta 3
• CENTB3
• DDEF2FLJ42910
• development and differentiation enhancing factor 2
• Development and differentiation-enhancing factor 2
• KIAA0400AMAP2
• Pap-alpha
• PAPPAG3
• Paxillin-associated protein with ARF GAP activity 3
• PYK2 C terminus-associated protein
• Pyk2 C-terminus-associated protein
• SHAG1

Background

This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ASAP2