PADI4 Antibody (PSH01-33)

Western Blot: PADI4 Antibody (PSH01-33) [NBP3-32707] - Western blot analysis of PADI4 on different lysates with Rabbit anti-PADI4 antibody (NBP3-32707) at 1/1,000 dilution. Lane 1: 293T overexpress PADI4 whole cell lysate Lane 2: 293T whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32707) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PADI4 Antibody (PSH01-33) [NBP3-32707] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-PADI4 antibody (NBP3-32707) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32707) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: PSH01-33
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PADI4 Antibody (PSH01-33) Summary

• Description: Novus Biologicals Rabbit PADI4 Antibody (PSH01-33) (NBP3-32707) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human PADI4 aa 1-663 / 663. (Uniprot: Q9UM07)
• Localization: Cytoplasm, Nucleus, Cytoplasmic granule.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PADI4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:1000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PADI4 Antibody (PSH01-33)

• HL-60 PAD
• PAD
• PAD4
• PADI4
• PADI5PADI-H protein
• PADPAD4
• PDI4
• PDI5EC 3.5.3.15
• peptidyl arginine deiminase, type IV
• peptidyl arginine deiminase, type V
• Peptidylarginine deiminase IV
• Protein-arginine deiminase type IV
• protein-arginine deiminase type-4

Background

In vivo expression of PADI4 correlated with the appearance of citrulline in histones. We have shown that PADI4 converts arginines in the histone H3 tail to citrulline, that PADI4 blocks transcriptional activation and is recruited to the endogenous promotor. PADI4 represents a novel class of histone modifying enzymes probably linked to eostrogen regulated transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PADI4