PA1 Antibody - BSA Free

Western Blot: PA1 Antibody [NBP1-06542] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-06542 used for IP at 3 ug/mg lysate. PA1 was also immunoprecipitated by NBP1-06541 which recognize upstream epitopes.

Immunohistochemistry: PA1 Antibody [NBP1-06542] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- PA1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: PA1 Antibody [NBP1-06542] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-PA1 used at a dilution of 1:250.

• Reactivity: Hu
• Applications: IHC, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PA1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PA1 Antibody - BSA Free (NBP1-06542) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 204 and 254 of human PTIP-associated 1 protein using the numbering given in entry NP_078792.1 (GeneID 79447).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAGR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 mcg/mg lysate
• Application Notes: This antibody is not recommended for Western Blot analysis.
• Control:

  PA1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PA1 Antibody - BSA Free

• chromosome 16 open reading frame 53
• FLJ22459
• GAS
• glutamate-rich coactivator interacting with SRC1/NCOA1
• MGC4606
• PA1
• PAXIP1-associated protein 1
• PTIP-associated 1 protein
• PTIP-associated protein 1

Background

C16ORF53 (PA1) is a component of a Set1-like multiprotein histone methyltransferase complex (Cho et al., 2007 [PubMed 17500065]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAGR1
• Entrez:
  • 79447(Human)
• Uniprot:
  • NP_078792.1(Human)