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p97/VCP Antibody (4A8)

Images

 
Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of VCP expression in Hela S3 NE.
Immunohistochemistry-Paraffin: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of monoclonal antibody to VCP on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml
Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - VCP monoclonal antibody (M03), clone 4A8. Western Blot analysis of VCP expression in HepG2 ( Cat # L019V1 ).
Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of VCP expression in PC-12.
Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of VCP expression in Raw 264.7.
ELISA: p97/VCP Antibody (4A8) [H00007415-M03] - Detection limit for recombinant GST tagged VCP is 1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clone
4A8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

p97/VCP Antibody (4A8) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
VCP (AAH07562, 1 a.a. ~ 588 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG
Specificity
VCP - valosin-containing protein (4A8)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
VCP
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Publications
Read Publications using H00007415-M03.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for p97/VCP Antibody (4A8)

  • 15S Mg(2+)-ATPase p97 subunit
  • IBMPFD
  • IBMPFDMGC8560
  • MGC131997
  • p97
  • TER ATPase
  • TERA
  • transitional endoplasmic reticulum ATPase
  • valosin containing protein
  • Valosin-containing proteinMGC148092
  • VCP
  • yeast Cdc48p homolog

Background

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p97/VCP Antibody (H00007415-M03)(3)

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Product General Protocols

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WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VCP
Entrez
Uniprot