p97/VCP Antibody (2B2) - Azide and BSA Free

Western Blot: p97/VCP Antibody (2B2) [H00007415-M18] - Analysis of VCP expression in A-431 (Cat # L015V1).

Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (2B2) [H00007415-M18] - Analysis of monoclonal antibody to VCP on HeLa cell. Antibody concentration 10 ug/ml

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

p97/VCP Antibody (2B2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: VCP (NP_009057.1, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKY
• Specificity: Reacts with valosin-containing protein.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VCP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Antibody reactivity against cell lysate in WB and recombinant protein in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for p97/VCP Antibody (2B2) - Azide and BSA Free

• 15S Mg(2+)-ATPase p97 subunit
• IBMPFD
• IBMPFDMGC8560
• MGC131997
• p97
• TER ATPase
• TERA
• transitional endoplasmic reticulum ATPase
• valosin containing protein
• Valosin-containing proteinMGC148092
• VCP
• yeast Cdc48p homolog

Background

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p97/VCP Antibody (H00007415-M18)(1)

Publication using H00007415-M18

• Ren W, Mohammed SI, Wereley S, Irudayaraj J. Magnetic Focus Lateral Flow Sensor for Detection of Cervical Cancer Biomarkers. Anal. Chem. 2019-01-29 [PMID: 30632735] (ELISA, Human)

Bioinformatics

• Gene Symbol: VCP
• Entrez:
  • 7415(Human)
• Uniprot:
  • NP_009057(Human)