p63/TP73L Antibody

Product Discontinued

p63/TP73L Antibody Summary

• Immunogen: A synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 of human p40
• Localization: Nuclear
• Marker: Squamous, Basal & Myoepithelial Cell Marker
• Specificity: p40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p63 antibody's ability to distinguish between the tumor types appears to be inferior when compared to p40. The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TP63
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2ug/ml
  • Immunohistochemistry 1-2ug/ml
  • Immunohistochemistry-Paraffin 1-2ug/ml
  • Western Blot 0.5-1ug/ml
• Application Notes: Human Chromosome Location: 3q28
  Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10mM PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for p63/TP73L Antibody

• amplified in squamous cell carcinoma
• Chronic ulcerative stomatitis protein
• CUSP
• EEC3
• EEC3B(p51B)
• Keratinocyte transcription factor KET
• KET
• KETRHS
• LMS
• OFC8
• OFC8SHFM4TP73LNBP
• p40
• p51
• p51tumor protein p53-like
• p53CP
• p63
• p63AIS
• p73H
• p73L
• p73LB(p51A)
• SHFM4
• TP53CP
• TP53L
• TP63
• TP73L
• Transformation-related protein 63
• tumor protein 63
• tumor protein p53-competing protein
• tumor protein p63 deltaN isoform delta
• tumor protein p63
• Tumor protein p73-like

Background

The ability to differentiate between lung adenocarcinoma vs squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p63 antibody's ability to distinguish between the tumor types appears to be inferior when compared to p40. The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future subclassification of lung cancers, especially by immunohistochemical techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p63/TP73L Antibody (NBP2-29467)(5)

Publications using NBP2-29467

• Park M, Richardson A, Delic N et al. A Bioengineering?Regenerative Medicine Approach for Ocular Surface Reconstruction Using a Functionalized Native Cornea?Derived Bio?Scaffold Advanced Functional Materials 2023-09-04 (ICC/IF, Mouse)
• Mollaoglu G, Jones A, Wait SJ et al. The Lineage-Defining Transcription Factors SOX2 and NkX2-1 Determine Lung Cancer Cell Fate and Shape the Tumor Immune Microenvironment. Immunity. 2018-10-16 [PMID: 30332632] (IHC-P, Human)
• Henry G, Malewska A, Mauck R et al. Molecular pathogenesis of human prostate basal cell hyperplasia. Prostate 2017-01-01 [PMID: 28795417] (IHC-P, Human)
• Yu M, Bojic S, Figueiredo GS et al. An important role for adenine, cholera toxin, hydrocortisone and triiodothyronine in the proliferation, self-renewal and differentiation of limbal stem cells in vitro. Exp. Eye Res. 2016-09-28 [PMID: 27693410] (IHC-P, Human)
• Schnittke N, Herrick DB, Lin B et al. Transcription factor p63 controls the reserve status but not the stemness of horizontal basal cells in the olfactory epithelium. Proc. Natl. Acad. Sci. U.S.A. 2015-09-08 [PMID: 26305958] (FLOW, WB, Mouse)

FAQs for p63/TP73L Antibody (NBP2-29467). (Showing 1 - 1 of 1 FAQs).

1. Could you please recommend positive cell control for p40 (p63 delta) Antibody (NBP2-29467)? Are HeLa cells positive for this marker?
   • Our own testing data for the p40 (p63 delta) antibody with catalogue number NBP2-29467 was produced using HEK293 cell lysate for Western blotting, and human lung squamous cell carcinoma for IHC. We do not currently have any other testing data for this antibody that I can share with you, and we have not yet evaluated it against HeLa lysate.

Bioinformatics

• Gene Symbol: TP63
• Uniprot:
  • Q7Z6M1(Human)