p62/SQSTM1 Antibody (2533B) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB, IHC, IP, ICC/IF, KO
• Clone: 2533B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 532

p62/SQSTM1 Antibody (2533B) [Alexa Fluor® 532] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant human p62/SQSTM1
  Asp368-Leu440
  Accession # Q13501
• Specificity: Detects human p62/SQSTM1 in direct ELISAs.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p62/SQSTM1 Antibody (2533B) [Alexa Fluor® 532]

• A170
• Autophagy Receptor P62
• DMRV
• EBI3-associated protein of 60 kDa
• EBI3-associated protein p60
• EBIAP
• FTDALS3
• NADGP
• ORCA
• OSIL
• oxidative stress induced like
• p60PDB3
• p62
• p62B
• Paget disease of bone 3
• PDB3
• phosphotyrosine independent ligand for the Lck SH2 domain p62
• Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
• Sequestosome 1
• sequestosome-1
• SQSTM1
• Ubiquitin-binding protein p62
• ZIP3

Background

SQSTM1 (Sequestrome-1), also called p62, is a widely expressed, stress-inducible, multifunctional 62 kDa intracellular protein. The 440 amino acid (aa) human SQSTM1 contains multiple adaptor domains that allow interaction with proteins in NGF/NFkB and other signaling pathways (notably TRAF6, atypical protein kinase C family and Src family), polyubiquitin, proteasome subunits and many others. It contains numerous regulatory phosphorylation sites and a dimerization site. SQSTM1 shuttles ubiquitinylated proteins to the proteasome and is important in autophagy and apoptosis. Its dysregulation is associated with Paget’s disease of bone, Parkinson’s and Alzheimer’s diseases, and cancers. Within aa 344-440, which includes the ubiquitin-binding domain, human SQSTM1 shares 100% aa sequence identity with mouse and rat SQSTM1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.