p53R2 Antibody

Western Blot: p53R2 Antibody [NBP2-19668] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.

Western Blot: p53R2 Antibody [NBP2-19668] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: p53R2 Antibody [NBP2-19668] - p53R2 antibody [N1C3] detects p53R2 protein at cytoplasm in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. p53R2 antibody [N1C3] (NBP2-19668) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

p53R2 Antibody Summary

• Description: Novus Biologicals Rabbit p53R2 Antibody (NBP2-19668) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human p53R2. The exact sequence is proprietary.
• Localization: Cytoplasm; Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RRM2B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for p53R2 Antibody

• EC 1.17.4.1
• MGC102856
• MGC42116
• MTDPS8A
• MTDPS8B
• p53-inducible ribonucleotide reductase small subunit 2 homolog
• p53-inducible ribonucleotide reductase small subunit 2-like protein
• p53R2
• P53R2DKFZp686M05248
• PEOA5
• ribonucleoside-diphosphate reductase subunit M2 B
• ribonucleotide reductase M2 B (TP53 inducible)
• RRM2B
• TP53-inducible ribonucleotide reductase M2 B

Background

This gene encodes the small subunit of a p53-inducible ribonucleotide reductase. This heterotetrameric enzyme catalyzes the conversion of ribonucleoside diphosphates to deoxyribonucleoside diphosphates. The product of this reaction is necessary for DNA synthesis. Mutations in this gene have been associated with autosomal recessive mitochondrial DNA depletion syndrome, autosomal dominant progressive external ophthalmoplegia-5, and mitochondrial neurogastrointestinal encephalopathy. Alternatively spliced transcript variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RRM2B
• Uniprot:
  • Q7LG56()